This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Cd6 antibody (A03913-2) performs well in ELISA, IHC and shows reactivity with Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IHC analysis of Cd6 using anti-Cd6 antibody (A03913-2).
Cd6 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd6 Antibody (A03913-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Cd6 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd6 Antibody (A03913-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-Cd6 Antibody
Cd6 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Rat | Rabbit | ELISA, IHC |
Tested Applications
Cd6 antibody was positively detected by IHC in:
rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Rat
ELISA
0.1-0.5 μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Cd6 Antibody
View all CD6 Antibodies
View all CD6 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat Cd6 recombinant protein (Position: S52-Q395).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of Cd6
CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-Cd6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03913-2)
Protocols
Immunohistochemistry (IHC) protocol for Cd6 antibody
Review
Have you used Anti-Cd6 Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Cd6 Antibody performs excellently in relevant applications for ELISA, IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A03913-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026