Anti-CD74 (B-Cell Marker) Monoclonal Antibody

Product Name

Anti-CD74 (B-Cell Marker) Monoclonal Antibody

View all CD74 Antibodies

SKU/Catalog Number

M01340-1

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD74 (B-Cell Marker) Monoclonal Antibody (Catalog # M01340-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Baboon, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD74 (B-Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01340-1)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: LN-2

Isotype

IgG1, kappa

Immunogen

SU-DHL-4 lymphoma cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01340-1 is reactive to CD74 in Human, Mouse, Baboon

Applications

M01340-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

33.516kDa

Background of CD74

This monoclonal antibody recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
(Methanol Fixed)Immunofluorescence (1-4ug/ml)
Western Blot (2-4ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD74 Mouse Monoclonal Antibody (LN-2).

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Formalin-fixed, paraffin-embedded human Spleen stained with Anti-CD74 Mouse Monoclonal Antibody (LN-2).

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Immunofluorescence analysis of Raji cells labeling CD74 with Anti-CD74 Mouse Monoclonal Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

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Flow Cytometric analysis of Human Raji cells using Anti-CD74 Mouse Monoclonal Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).

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Western blot analysis of Raji cell lysate using Anti-CD74 Mouse Monoclonal Antibody (LN-2).

Gene/Protein Information For CD74 (Source: Uniprot.org, NCBI)

Alternative Names

CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated); CD74 antigen; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74; CLIP; DHLAG; DHLAGgamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-gamma; Ii; INVG34; MHC HLA-DR gamma chain; p33 CD74 DHLAG, HLADG, II, Ia-GAMMA, p33 CD74 molecule HLA class II histocompatibility antigen gamma chain|CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)|CD74 molecule, major histocompatibility complex, class II invariant chain|HLA-DR antigens-associated invariant chain|HLA-DR-gamma|Ia-associated invariant chain|MHC HLA-DR gamma chain|gamma chain of class II antigens

For more info on CD74, check out the CD74 Infographic

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In this infographic, you will see the following information for CD74: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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