Anti-CD90/Thy1 Antibody

THY1 antibody

Boster Bio Anti-CD90/Thy1 Antibody catalog # PA1515-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 7 publication(s).

Product Info Summary

SKU: PA1515-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-CD90/Thy1 Antibody

View all THY1 Antibodies

SKU/Catalog Number

PA1515-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD90/Thy1 Antibody catalog # PA1515-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD90/Thy1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1515-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CD90, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1515-1 is reactive to THY1 in Human, Mouse, Rat

Applications

PA1515-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

17.935kDa

Background of THY1

CD90 (Cluster of Differentiation 90) or Thy-1 is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. The CD90 gene is mapped to 11q23.3. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For THY1 (Source: Uniprot.org, NCBI)

Gene Name

THY1

Full Name

Thy-1 membrane glycoprotein

Weight

17.935kDa

Alternative Names

CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1 THY1 CD90, CDw90 Thy-1 cell surface antigen thy-1 membrane glycoprotein|Thy-1 T-cell antigen|thy-1 antigen

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on THY1, check out the THY1 Infographic

THY1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for THY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1515-1 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
PA1515-1 usage in article: APP:WB, SAMPLE:MSC, DILUTION:NA

Therapeutic efficacy of neural stem cells originating from umbilical cord-derived mesenchymal stem cells in diabetic retinopathy

Differentiation of mesenchymal stem cell in the microenviroment of retinitis pigmentosa

Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.

Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells

Li F, Xu Y, Xu X, Xu B, Zhao J, Zhang X. Mol Med Rep. 2014 Jul;10(1):322-8. Doi: 10.3892/Mmr.2014.2220. Epub 2014 May 8. Fms-Related Tyrosine Kinase??3 Ligand Promotes Proliferation Of Placenta Amnion And Chorion Mesenchymal Stem Cells In??vitro.

Huang Y, Jia X, Bai K, Gong X, Fan Y. Arch Med Res. 2010 Oct;41(7):497-505. Doi: 10.1016/J.Arcmed.2010.10.002. Effect Of Fluid Shear Stress On Cardiomyogenic Differentiation Of Rat Bone Marrow Mesenchymal Stem Cells.

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4 Customer Q&As for Anti-CD90/Thy1 Antibody

Question

We are currently using anti-CD90/Thy1 antibody PA1515-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

The anti-CD90/Thy1 antibody (PA1515-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-13

Question

We have seen staining in human anterior cingulate cortex. Do you have any suggestions? Is anti-CD90/Thy1 antibody supposed to stain anterior cingulate cortex positively?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

From what I have seen in literature anterior cingulate cortex does express THY1. From what I have seen in Uniprot.org, THY1 is expressed in anterior cingulate cortex, brain, amygdala, eye, liver, leukemic t-cell, among other tissues. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2019-12-05

Question

Our lab were well pleased with the WB result of your anti-CD90/Thy1 antibody. However we have observed positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is THY1 supposed to be expressed?

L. Anderson

Verified customer

Asked: 2018-08-30

Answer

Based on literature, leukemic t-cell does express THY1. Generally THY1 expresses in cell membrane. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218

Boster Scientific Support

Answered: 2018-08-30

Question

I am interested in using your anti-CD90/Thy1 antibody for t cell receptor signaling pathway studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

C. Johnson

Verified customer

Asked: 2013-02-21

Answer

We appreciate your inquiry. This PA1515-1 anti-CD90/Thy1 antibody is validated on rat thymus tissue, brain tissue, tissue lysate, liver tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-02-21

Order DetailsPrice
PA1515-1

100μg

$370
PA1515-1-10ug

10μg sample (liquid)

$99
PA1515-1-Biotin

100 μg Biotin conjugated

$570
PA1515-1-Cy3

100 μg Cy3 conjugated

$570
PA1515-1-Dylight488

100 μg Dylight488 conjugated

$570
PA1515-1-Dylight550

100 μg Dylight550 conjugated

$570
PA1515-1-Dylight594

100 μg Dylight594 conjugated

$570
PA1515-1-FITC

100 μg FITC conjugated

$570
PA1515-1-HRP

100 μg HRP conjugated

$570
PA1515-1-APC

100 μg APC conjugated

$670
PA1515-1-PE

100 μg PE conjugated

$670
PA1515-1-carrier-free

Carrier Free

$370

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PA1515-1
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