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Product Info Summary
| SKU: | M00382 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Cdc20 (Cell Division Cycle Protein 20) Monoclonal Antibody
SKU/Catalog Number
M00382
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Cdc20 (Cell Division Cycle Protein 20) Monoclonal Antibody (Catalog # M00382). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Cdc20 (Cell Division Cycle Protein 20) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00382)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: CDC20/1102
Isotype
IgG1, kappa
Immunogen
Recombinant human Cdc20 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00382 is reactive to CDC20 in Human
Applications
M00382 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.723kDa
Background of CDC20
Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with 1ml water (15 minutes at room temperature).
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Gastric carcinoma stained with Anti-CDC20 Monoclonal Antibody (CDC20/1102).
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CDC20 Monoclonal Antibody (CDC20/1102)
Protein Target Info & Infographic
Gene/Protein Information For CDC20 (Source: Uniprot.org, NCBI)
Gene Name
CDC20
Full Name
Cell division cycle protein 20 homolog
Weight
54.723kDa
Superfamily
WD repeat CDC20/Fizzy family
Alternative Names
CDC20 cell division cycle 20 homolog (S. cerevisiae); CDC20 cell division cycle 20 homolog; cell division cycle 20 homolog (S. cerevisiae); cell division cycle protein 20 homolog; MGC102824; p55CDCS. cerevisiae, homolog) CDC20 CDC20A, bA276H19.3, p55CDC cell division cycle 20 cell division cycle protein 20 homolog|CDC20 cell division cycle 20 homolog|cell division cycle 20 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC20, check out the CDC20 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cdc20 (Cell Division Cycle Protein 20) Monoclonal Antibody (M00382)
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No publications found for M00382
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