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Product Info Summary
| SKU: | M00159-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-CDK4 Antibody
SKU/Catalog Number
M00159-2
Size
50 µl
Description
Boster Bio Anti-CDK4 Antibody (Catalog # M00159-2). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CDK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00159-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1529CT850.162.73
Isotype
IgG2a,κ
Immunogen
This CDK4 antibody is generated from a mouse immunized with a recombinant protein.
Reactive Species
M00159-2 is reactive to CDK4 in Human, Mouse
Calculated molecular weight
33.7 kDa
Background of CDK4
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00159-2 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC: 1:300
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human Breast cancer section using Pink1. M00159-2 was diluted at 1:300 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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All lanes : Anti-CDK4 Antibody at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 34 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M00159-2 staining CDK4 in human breast carcinoma sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-CDK4 Antibody (M00159-2)
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