Anti-CDK4 Rabbit Monoclonal Antibody

CDK4 antibody

Boster Bio Anti-CDK4 Rabbit Monoclonal Antibody catalog # M00159-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: M00159-1
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-CDK4 Rabbit Monoclonal Antibody

View all CDK4 Antibodies

SKU/Catalog Number

M00159-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CDK4 Rabbit Monoclonal Antibody catalog # M00159-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDK4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00159-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GIC-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CDK4

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00159-1 is reactive to CDK4 in Human

Applications

M00159-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

33.73kDa

Background of CDK4

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CDK4 (Source: Uniprot.org, NCBI)

Gene Name

CDK4

Full Name

Cyclin-dependent kinase 4

Weight

33.73kDa

Superfamily

protein kinase superfamily

Alternative Names

CDK4; Cell division protein kinase 4; CMM3; cyclin-dependent kinase 4; EC 2.7.11; EC 2.7.11.22; melanoma cutaneous malignant, 3; MGC14458; PSK-J3; PSK-J3cell division kinase 4 CDK4 CMM3, PSK-J3 cyclin dependent kinase 4 cyclin-dependent kinase 4|cell division protein kinase 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDK4, check out the CDK4 Infographic

CDK4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00159-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of Bone Morphogenetic Protein-2 on Proliferation and Apoptosis of Gastric Cancer Cells

Zhang H, Zhao B, Huang C, Meng Xm, Bian Eb, Li J. Plos One. 2014 May 2;9(5):E95520. Doi: 10.1371/Journal.Pone.0095520. Ecollection 2014. Melittin Restores Pten Expression By Down-Regulating Hdac2 In Human Hepatocelluar Carcinoma Hepg2 Cells.

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16 Customer Q&As for Anti-CDK4 Rabbit Monoclonal Antibody

Question

Is this M00159-1 anti-CDK4 Rabbit Monoclonal antibody reactive to the isotypes of CDK4?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

The immunogen of M00159-1 anti-CDK4 Rabbit Monoclonal antibody is A synthesized peptide derived from human CDK4 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-30

Question

I see that the anti-CDK4 Rabbit Monoclonal antibody M00159-1 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-14

Question

We are currently using anti-CDK4 Rabbit Monoclonal antibody M00159-1 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

The anti-CDK4 Rabbit Monoclonal antibody (M00159-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-19

Question

We want to test anti-CDK4 Rabbit Monoclonal antibody M00159-1 on human right ovary for research purposes, then I may be interested in using anti-CDK4 Rabbit Monoclonal antibody M00159-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

The products we sell, including anti-CDK4 Rabbit Monoclonal antibody M00159-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-10

Question

Can you help my question with product M00159-1, anti-CDK4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00159-1 anti-CDK4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-27

Question

Does anti-CDK4 Rabbit Monoclonal antibody M00159-1 work for IF with right ovary?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

According to the expression profile of right ovary, CDK4 is highly expressed in right ovary. So, it is likely that anti-CDK4 Rabbit Monoclonal antibody M00159-1 will work for IF with right ovary.

Boster Scientific Support

Answered: 2020-01-20

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-CDK4 Rabbit Monoclonal antibody M00159-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-06

Question

Is a blocking peptide available for product anti-CDK4 Rabbit Monoclonal antibody (M00159-1)?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

We do provide the blocking peptide for product anti-CDK4 Rabbit Monoclonal antibody (M00159-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-11

Question

We bought anti-CDK4 Rabbit Monoclonal antibody for IF on muscle last year. I am using human, and We want to use the antibody for WB next. I would like examining muscle as well as embryo in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

I looked at the website and datasheets of our anti-CDK4 Rabbit Monoclonal antibody and I see that M00159-1 has been validated on human in both IF and WB. Thus M00159-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-25

Question

We have observed staining in human right ovary. Do you have any suggestions? Is anti-CDK4 Rabbit Monoclonal antibody supposed to stain right ovary positively?

A. Collins

Verified customer

Asked: 2019-06-27

Answer

According to literature right ovary does express CDK4. According to Uniprot.org, CDK4 is expressed in right ovary, embryo, muscle, among other tissues. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-27

Question

Will M00159-1 anti-CDK4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

As indicated on the product datasheet, M00159-1 anti-CDK4 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-15

Question

Is there a BSA free version of anti-CDK4 Rabbit Monoclonal antibody M00159-1 available?

Verified Customer

Verified customer

Asked: 2018-08-08

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDK4 Rabbit Monoclonal antibody M00159-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-08-08

Question

I was wanting to use your anti-CDK4 Rabbit Monoclonal antibody for IF for human right ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human right ovary identification?

Verified Customer

Verified customer

Asked: 2018-06-21

Answer

You can see on the product datasheet, M00159-1 anti-CDK4 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right ovary in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-21

Question

We are interested in using your anti-CDK4 Rabbit Monoclonal antibody for regulation of insulin receptor signaling pathway studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-10-24

Answer

Thanks for your inquiry. This M00159-1 anti-CDK4 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-10-24

Question

I have attached the WB image, lot number and protocol we used for right ovary using anti-CDK4 Rabbit Monoclonal antibody M00159-1. Please let me know if you require anything else.

J. Evans

Verified customer

Asked: 2017-05-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-17

Question

Our team were well pleased with the WB result of your anti-CDK4 Rabbit Monoclonal antibody. However we have observed positive staining in muscle cytoplasm. using this antibody. Is that expected? Could you tell me where is CDK4 supposed to be expressed?

A. Dhar

Verified customer

Asked: 2015-12-14

Answer

From literature, muscle does express CDK4. Generally CDK4 expresses in cytoplasm. Regarding which tissues have CDK4 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 14702039
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2015-12-14

Order DetailsPrice
M00159-1

100μL

$370
M00159-1-30ul

30μL sample

$99
M00159-1-carrier-free

Carrier Free

$370

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M00159-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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