Anti-Cyclin-dependent-like kinase 5 Cdk5 Antibody

Product Name

Anti-Cyclin-dependent-like kinase 5 Cdk5 Antibody

View all CDK5 Antibodies

SKU/Catalog Number

A00511

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Cyclin-dependent-like kinase 5 Cdk5 Antibody (Catalog # A00511). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Cyclin-dependent-like kinase 5 Cdk5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00511)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

CDK5 (p31) peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00511 is reactive to CDK5 in Human, Mouse, Rat

Observed Molecular Weight

39 kDa

Calculated molecular weight

33.3 kDa

Background of CDK5

The protein encoded by the gene CDK5 is involved in the control of the cell cycle and interacts with D1 and D3-type G1 cyclins. CDK5 belongs to the protein kinase superfamily, the CMGC Ser/Thr protein kinase family and the CDC2/CDKX subfamily. CDK5 phosphorylates histone H1, tau, MAP2 and NF-H and NF-M and also interacts with p35 which activates the kinase. CDK5 forms a heterodimer, where CDK5 is the catalytic subunit and p35 is the regulatory subunit. CDK5 is also found in a trimolecular complex with CABLES1 and ABL1. CDK5 is a cytoplasmic protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00511 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IHC: 1:500
IP: 1:100
WB: 1:500 - 1:5,000
CDK5 antibody was tested by immunohistochemistry and immunoblot and found to be reactive against cdk5 (p31) at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  Anti-cdk5 (p31) is suitable for the detection by immunoblot of human, rat and mouse cdk5 (p31).  Suggested dilutions for ELISA are 1:500 to 1:2000.

Validation Images & Assay Conditions

See full target information CDK5

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis