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Product Info Summary
| SKU: | A00016-5 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p16INK4a/CDKN2A Antibody
SKU/Catalog Number
A00016-5
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-p16INK4a/CDKN2A Antibody catalog # A00016-5. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-p16INK4a/CDKN2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00016-5)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human P16-INK4A/CDKN2A recombinant protein (Position: P48-D156).
Reactive Species
A00016-5 is reactive to CDKN2A in Human
Calculated molecular weight
16.5 kDa
Background of CDKN2A
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00016-5 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400
Immunoprecipitation, 1:50
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Western blot analysis of p16INK4a/CDKN2A using anti-p16INK4a/CDKN2A antibody (A00016-5).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p16INK4a/CDKN2A antigen affinity purified polyclonal antibody (A00016-5) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for p16INK4a/CDKN2A at approximately 17 kDa. The expected band size for p16INK4a/CDKN2A is at 17 kDa.
Specific Publications For Anti-p16INK4a/CDKN2A Antibody (A00016-5)
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