Product Info Summary
SKU: | A00356-1 |
---|---|
Size: | 80 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, WB, IHC-P-Leica |
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Product info
Product Name
Anti-CEA Antibody (Center)
View all CEACAM5/CD66e Antibodies
SKU/Catalog Number
A00356-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-CEA Antibody (Center) (Catalog # A00356-1). Tested in IHC-P-Leica, Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CEA Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00356-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CEA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-429 amino acids from the Central region of human CEA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00356-1 is reactive to CEACAM5 in Human
Applications
A00356-1 is guaranteed for Flow Cytometry, WB, IHC-P-Leica Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
76.795kDa
Background of CEACAM5/CD66e
CEA is a cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. It is a receptor for E.coli Dr adhesins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P-Leica: 1:500
FC: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes : Anti-CEA Antibody (Center) at 1:1000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: HT-29 whole cell lysate
Lane 3: PC-3 whole cell lysate
Lane 4: A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 77 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue using A00356-1 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Click image to see more details
CEA Antibody (Center) (Cat. #A00356-1) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For CEACAM5 (Source: Uniprot.org, NCBI)
Gene Name
CEACAM5
Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
Weight
76.795kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100 CEACAM5 CD66e, CEA CEA cell adhesion molecule 5 carcinoembryonic antigen-related cell adhesion molecule 5|carcinoembryonic antigen related cell adhesion molecule 5|meconium antigen 100
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEACAM5, check out the CEACAM5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEACAM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CEA Antibody (Center) (A00356-1)
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No publications found for A00356-1
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