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Product Info Summary
| SKU: | A00356-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB, IHC-P-Leica |
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Product info
Product Name
Anti-CEA Antibody (Center)
SKU/Catalog Number
A00356-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-CEA Antibody (Center) (Catalog # A00356-1). Tested in IHC-P-Leica, Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CEA Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00356-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CEA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-429 amino acids from the Central region of human CEA.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00356-1 is reactive to CEACAM5 in Human
Calculated molecular weight
76.8 kDa
Background of CEACAM5
CEA is a cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. It is a receptor for E.coli Dr adhesins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00356-1 is guaranteed for Flow Cytometry, WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P-Leica: 1:500
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-CEA Antibody (Center) at 1:1000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: HT-29 whole cell lysate
Lane 3: PC-3 whole cell lysate
Lane 4: A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 77 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue using A00356-1 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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CEA Antibody (Center) (Cat. #A00356-1) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-CEA Antibody (Center) (A00356-1)
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