Anti-Choline Acetyltransferase/CHAT Antibody Picoband™

Product Name

Anti-Choline Acetyltransferase/CHAT Antibody Picoband™

View all Choline Acetyltransferase/ChAT Antibodies

SKU/Catalog Number

A01192-6

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Choline Acetyltransferase/CHAT Antibody Picoband™ catalog # A01192-6. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Choline Acetyltransferase/CHAT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01192-6)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Choline Acetyltransferase/CHAT recombinant protein (Position: L131-S704).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01192-6 is reactive to CHAT in Human, Mouse, Rat

Applications

A01192-6 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

71 kDa

Calculated molecular weight

82.536kDa

Background of Choline Acetyltransferase/ChAT

Choline acetyltransferase(commonly abbreviated asChAT, but sometimesCAT) is atransferaseenzymeresponsible for the synthesis of theneurotransmitteracetylcholine. In humans, the choline acetyltransferase enzyme is encoded by theCHATgene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Choline Acetyltransferase/CHAT using anti-Choline Acetyltransferase/CHAT antibody (A01192-6).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Choline Acetyltransferase/CHAT antigen affinity purified polyclonal antibody (Catalog # A01192-6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Choline Acetyltransferase/CHAT at approximately 71 kDa. The expected band size for Choline Acetyltransferase/CHAT is at 71 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of U937 cells using anti-Choline Acetyltransferase/CHAT antibody (A01192-6).
Overlay histogram showing U937 cells stained with A01192-6 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Choline Acetyltransferase/CHAT Antibody (A01192-6, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CHAT (Source: Uniprot.org, NCBI)

Alternative Names

acetyl CoA:choline O-acetyltransferase; CHAT; CHOACTase; Choline acetylase; Choline Acetyltransferase; choline O-acetyltransferase; CMS1A; CMS1A2; EC 2.3.1; EC 2.3.1.6 CHAT CHOACTASE, CMS1A, CMS1A2, CMS6 choline O-acetyltransferase choline O-acetyltransferase|acetyl CoA:choline O-acetyltransferase|choline acetylase

For more info on CHAT, check out the CHAT Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CHAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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