Anti-CIP75 UBQLN4 Antibody

Product Name

Anti-CIP75 UBQLN4 Antibody

View all UBQLN4 Antibodies

SKU/Catalog Number

A08887-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CIP75 UBQLN4 Antibody (Catalog # A08887-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CIP75 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CIP75 UBQLN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08887-1)

Host

Rabbit

Contents

CIP75 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CIP75 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CIP75. The immunogen is located within the first 50 amino acids of CIP75.

Reactive Species

A08887-1 is reactive to UBQLN4 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

63.9 kDa

Background of UBQLN4

The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08887-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CIP75 antibody can be used for detection of CIP75 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information UBQLN4

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