Product Info Summary
SKU: | A03134-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Clathrin heavy chain/CLTC Antibody Picoband™
View all Clathrin Heavy Chain 1/CHC17 Antibodies
SKU/Catalog Number
A03134-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Clathrin heavy chain/CLTC Antibody Picoband™ catalog # A03134-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Clathrin heavy chain/CLTC Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03134-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Clathrin heavy chain/CLTC recombinant protein (Position: R967-Q1668).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03134-1 is reactive to CLTC in Human, Mouse, Rat
Applications
A03134-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
192 kDa
Calculated molecular weight
191.615kDa
Background of Clathrin Heavy Chain 1/CHC17
Clathrin heavy chain 1 is a protein that in humans is encoded by the CLTC gene. Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody (A03134-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse ANA-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Clathrin heavy chain/CLTC antigen affinity purified polyclonal antibody (Catalog # A03134-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Clathrin heavy chain/CLTC at approximately 192KD. The expected band size for Clathrin heavy chain/CLTC is at 192KD.
Click image to see more details
Figure 2. IHC analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody (A03134-1).
Clathrin heavy chain/CLTC was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Clathrin heavy chain/CLTC Antibody (A03134-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody (A03134-1).
Clathrin heavy chain/CLTC was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Clathrin heavy chain/CLTC Antibody (A03134-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody (A03134-1).
Clathrin heavy chain/CLTC was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Clathrin heavy chain/CLTC Antibody (A03134-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody (A03134-1).
Clathrin heavy chain/CLTC was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Clathrin heavy chain/CLTC Antibody (A03134-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of U87 cells using anti-Clathrin heavy chain/CLTC antibody (A03134-1).
Overlay histogram showing U87 cells stained with A03134-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Clathrin heavy chain/CLTC Antibody (A03134-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 7. Flow Cytometry analysis of HEPA1-6 cells using anti-Clathrin heavy chain/CLTC antibody (A03134-1).
Overlay histogram showing HEPA1-6 cells stained with A03134-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Clathrin heavy chain/CLTC Antibody (A03134-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 8. Flow Cytometry analysis of C6 cells using anti-Clathrin heavy chain/CLTC antibody (A03134-1).
Overlay histogram showing C6 cells stained with A03134-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Clathrin heavy chain/CLTC Antibody (A03134-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CLTC (Source: Uniprot.org, NCBI)
Gene Name
CLTC
Full Name
Clathrin heavy chain 1
Weight
191.615kDa
Superfamily
clathrin heavy chain family
Alternative Names
CHC; CHC17; Clathrin Heavy Chain 1; Clathrin heavy chain on chromosome 17; clathrin, heavy chain (Hc); clathrin, heavy chain; clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; CLH17; CLH-17; CLTC; CLTCL2; Hc; KIAA0034 CLTC CHC, CHC17, CLH-17L2, Hc, MRD56, CLTC clathrin heavy chain clathrin heavy chain 1|clathrin heavy chain on chromosome 17|clathrin, heavy polypeptide (Hc)|clathrin, heavy polypeptide-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLTC, check out the CLTC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLTC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Clathrin heavy chain/CLTC Antibody Picoband™ (A03134-1)
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