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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-Claudin 1 CLDN1 Rabbit Monoclonal Antibody
Boster Bio Anti-Claudin 1 CLDN1 Rabbit Monoclonal Antibody catalog # M01585. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin 1 CLDN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01585)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human Claudin 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01585 is reactive to CLDN1 in Human, Mouse, Rat
M01585 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Western blot analysis of Claudin 1 expression in A431 cell lysate (M01585).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLDN1 monoclonal antibody (Catalog # M01585) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLDN1
Gene/Protein Information For CLDN1 (Source: Uniprot.Org, NCBI)
claudin 1; Claudin1; Claudin-1; CLD1; CLDN1; ILVASCSenescence-associated epithelial membrane protein; SEMP1; SEMP1senescence-associated epithelial membrane protein 1 CLDN1 CLD1, ILVASC, SEMP1 claudin 1 claudin-1|senescence-associated epithelial membrane protein 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CLDN1, check out the CLDN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CLDN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M01585 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xiaozhu Tian,Rong Li,Yiming Jiang,Fei Zhao,Zhengsheng Yu,Yiqing Wang,Zixing Dong,Pu Liu,Xiangkai Li,Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake,J
M01585 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND ILEUM, DILUTION:NA
Xin-li Liang,Miao-miao Ji,Lai Chen,Ye Liao,Xiao-qiang Kong,Xi-qiang Xu,Zheng-gen Liao,Danny W. Wilson, Traditional Chinese herbal medicine Astragalus Radix and its effects on intestinal absorption of aconite alkaloids in rats, Chinese Herbal Medicines,202
M01585 usage in article: APP:WB, SAMPLE:DUODENAL TISSUE, DILUTION:NA
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