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Product Info Summary
| SKU: | M01585 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Claudin 1 CLDN1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01585
BM5140 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Claudin 1 CLDN1 Rabbit Monoclonal Antibody catalog # M01585. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin 1 CLDN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01585)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABEH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Claudin 1
Reactive Species
M01585 is reactive to CLDN1 in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
22.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01585 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: human A431 whole cell, human Caco-2 whole cell, human A549 whole cell, human HepG2 whole cell, rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Oral administration of SA-Q-CS MPs improves the levels of colonic tight-junction proteins. (A) IHC staining images of ZO-1 protein, black arrows indicate positive signal of ZO-1 protein (scale bar 100 μm); (B) Protein bands of Occludin, Claudin-1 and ZO-1; (C) IOD was counted of ZO-1 by immunohistochemical staining; (D) FITC fluorescence intensity in blood of C57/BL6 mice in each FITC group; ( E–G ) Protein statistical analysis of proteins Occludin, Claudin-1, and ZO-1. Data was represented as mean ± SD ( * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 by one-way ANOVA with Dunnett’s multiple comparisons test, n ≥ 3).
Index in PubMed under a CC BY license. PMID: 40842836
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Western blot analysis of Claudin 1 using anti-Claudin 1 antibody (M01585).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Claudin 1 antigen affinity purified monoclonal antibody (Catalog # M01585) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 1 at approximately 23 kDa. The expected band size for Claudin 1 is at 23 kDa.
Specific Publications For Anti-Claudin 1 CLDN1 Rabbit Monoclonal Antibody (M01585)
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Customer Q&As
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6 Customer Q&As for Anti-Claudin 1 CLDN1 Rabbit Monoclonal Antibody
Question
We are currently using anti-Claudin 1 Rabbit Monoclonal antibody M01585 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The anti-Claudin 1 Rabbit Monoclonal antibody (M01585) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-03
Question
My lab would like to test anti-Claudin 1 Rabbit Monoclonal antibody M01585 on mouse urinary bladder for research purposes, then I may be interested in using anti-Claudin 1 Rabbit Monoclonal antibody M01585 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
The products we sell, including anti-Claudin 1 Rabbit Monoclonal antibody M01585, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-26
Question
Will anti-Claudin 1 Rabbit Monoclonal antibody M01585 work for WB with urinary bladder?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
According to the expression profile of urinary bladder, CLDN1 is highly expressed in urinary bladder. So, it is likely that anti-Claudin 1 Rabbit Monoclonal antibody M01585 will work for WB with urinary bladder.
Boster Scientific Support
Answered: 2019-02-21
Question
Is there a BSA free version of anti-Claudin 1 Rabbit Monoclonal antibody M01585 available?
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Claudin 1 Rabbit Monoclonal antibody M01585 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urinary bladder using anti-Claudin 1 Rabbit Monoclonal antibody M01585. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-26
Question
I see that the anti-Claudin 1 Rabbit Monoclonal antibody M01585 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-09-12
