Click image to see more details
Product Info Summary
| SKU: | M00080-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-cleaved Caspase-9 p35 CASP9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00080-2
BM4521 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-cleaved Caspase-9 CASP9 Rabbit Monoclonal Antibody catalog # M00080-2. Tested in WB, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-cleaved Caspase-9 p35 CASP9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00080-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FEE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Caspase-9
Reactive Species
M00080-2 is reactive to CASP9 in Human, Mouse
Observed Molecular Weight
46 kDa
Calculated molecular weight
46.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00080-2 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Validation Images & Assay Conditions
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Western blot analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (M00080-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human Jurkat treated with staurosporine,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse NIH/3T3 treated with staurosporine.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 9/CASP9 antigen affinity purified monoclonal antibody (M00080-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 9/CASP9 at approximately 46 kDa. The expected band size for Caspase 9/CASP9 is at 46 kDa.
Specific Publications For Anti-cleaved Caspase-9 p35 CASP9 Rabbit Monoclonal Antibody (M00080-2)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-cleaved Caspase-9 p35 CASP9 Rabbit Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-01
Question
Is this M00080-2 anti-cleaved Caspase-9 Rabbit Monoclonal antibody reactive to the isotypes of CASP9?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
The immunogen of M00080-2 anti-cleaved Caspase-9 Rabbit Monoclonal antibody is A synthesized peptide derived from human cleaved Caspase-9. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-10
Question
I see that the anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-28
Question
We are interested in using your anti-cleaved Caspase-9 Rabbit Monoclonal antibody for intrinsic apoptotic signaling pathway in response to dna damage studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
I appreciate your inquiry. This M00080-2 anti-cleaved Caspase-9 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-16
Question
Would M00080-2 anti-cleaved Caspase-9 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
It shows on the product datasheet, M00080-2 anti-cleaved Caspase-9 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-31
Question
I was wanting to use your anti-cleaved Caspase-9 Rabbit Monoclonal antibody for IP for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
It shows on the product datasheet, M00080-2 anti-cleaved Caspase-9 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-18
Question
Is a blocking peptide available for product anti-cleaved Caspase-9 Rabbit Monoclonal antibody (M00080-2)?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
We do provide the blocking peptide for product anti-cleaved Caspase-9 Rabbit Monoclonal antibody (M00080-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-18
Question
We bought anti-cleaved Caspase-9 Rabbit Monoclonal antibody for WB on stomach cancer in a previous project. I am using mouse, and We intend to use the antibody for IP next. I am interested in examining stomach cancer as well as adrenal tissue in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
I have checked the website and datasheets of our anti-cleaved Caspase-9 Rabbit Monoclonal antibody and it appears that M00080-2 has been validated on mouse in both WB and IP. Thus M00080-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-04-11
Question
We are currently using anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2 for mouse tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
A. Jackson
Verified customer
Asked: 2018-01-12
Answer
The anti-cleaved Caspase-9 Rabbit Monoclonal antibody (M00080-2) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-12
Question
Would anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2 work for IP with cervix carcinoma erythroleukemia?
P. Anderson
Verified customer
Asked: 2017-12-20
Answer
According to the expression profile of cervix carcinoma erythroleukemia, CASP9 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2 will work for IP with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2017-12-20
Question
I am looking for to test anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
The products we sell, including anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-21
Question
We have observed staining in human cervix carcinoma. Any tips? Is anti-cleaved Caspase-9 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
D. Zhao
Verified customer
Asked: 2017-08-09
Answer
From literature cervix carcinoma does express CASP9. From Uniprot.org, CASP9 is expressed in adrenal tissue, t-cell, stomach cancer, kidney, eye lymph, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP9 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, and Lymph, Pubmed ID: 15489334
Kidney, Pubmed ID: 14702039
Stomach cancer, Pubmed ID: 9890966
T-cell, Pubmed ID: 8900201
Boster Scientific Support
Answered: 2017-08-09
Question
My question regarding product M00080-2, anti-cleaved Caspase-9 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Krishna
Verified customer
Asked: 2017-01-30
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00080-2 anti-cleaved Caspase-9 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-30
Question
Do you have a BSA free version of anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2 available?
V. Wu
Verified customer
Asked: 2014-10-29
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-cleaved Caspase-9 Rabbit Monoclonal antibody M00080-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-10-29
