Product Info Summary
SKU: | A01017-4 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-CNPase/CNP Antibody Picoband™
SKU/Catalog Number
A01017-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CNPase/CNP Antibody Picoband™ catalog # A01017-4. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CNPase/CNP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01017-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CNPase/CNP recombinant protein (Position: M1-I421).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01017-4 is reactive to CNP in Human, Mouse, Rat
Applications
A01017-4 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
47.579kDa
Background of CNPase
2',3'-Cyclic-nucleotide 3'-phosphodiesterase, also known as CNPase, is an enzyme that in humans is encoded by the CNP gene. And this gene is mapped to 17q21.2. CNPase is named for its ability to catalyze the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides. CNPase is thought to play a critical role in the events leading up to myelination. Additionally, CNPase has been demonstrated to inhibit the replication of HIV-1 and other primate lentiviruses by binding the retroviral Gag protein and inhibiting the genesis of nascent viral particles.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Mouse, Rat
Immunofluorescence, 5 µg/ml, Mouse, Rat
Flow Cytometry, 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysate,
Lane 7: mouse brain tissue lysate,
Lane 8: mouse Neuro-2a whole cell lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNPase/CNP antigen affinity purified polyclonal antibody (Catalog # A01017-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNPase/CNP at approximately 45 kDa. The expected band size for CNPase/CNP is at 45 kDa.
Click image to see more details
Figure 2. IHC analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4).
CNPase/CNP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CNPase/CNP Antibody (A01017-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4).
CNPase/CNP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CNPase/CNP Antibody (A01017-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4).
CNPase/CNP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CNPase/CNP Antibody (A01017-4) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. IF analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4).
CNPase/CNP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CNPase/CNP Antibody (A01017-4) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of U937 cells using anti-CNPase/CNP antibody (A01017-4).
Overlay histogram showing U937 cells stained with A01017-4 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CNPase/CNP Antibody (A01017-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CNP (Source: Uniprot.org, NCBI)
Gene Name
CNP
Full Name
2',3'-cyclic-nucleotide 3'-phosphodiesterase
Weight
47.579kDa
Superfamily
2H phosphoesterase superfamily
Alternative Names
2', 3' cyclic nucleotide 3' phosphohydrolase; 2'-3'-cyclic nucleotide 3' phosphodiesterase; 2'-3'-cyclic-nucleotide 3'-phosphodiesterase; CNP1; CNPase; EC 3.1.4.37 CNP CNP1, HLD20 2,3-cyclic nucleotide 3 phosphodiesterase 2,3-cyclic-nucleotide 3-phosphodiesterase|2, 3 cyclic nucleotide 3 phosphohydrolase|CNPase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNP, check out the CNP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CNPase/CNP Antibody Picoband™ (A01017-4)
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