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Product Info Summary
| SKU: | A06685 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC |
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Product info
Product Name
Anti-Collagen alpha-1(XIV) chain COL14A1 Antibody
SKU/Catalog Number
A06685
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Collagen alpha-1 (XIV) chain COL14A1 Antibody catalog # A06685. Tested in IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen alpha-1(XIV) chain COL14A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06685)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human c-ternal protein at AA range: 1571-1620
Reactive Species
A06685 is reactive to COL14A1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
193.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06685 is guaranteed for ELISA, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:50-200
ELISA 1:10000-20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:200.
Specific Publications For Anti-Collagen alpha-1(XIV) chain COL14A1 Antibody (A06685)
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5 Customer Q&As for Anti-Collagen alpha-1(XIV) chain COL14A1 Antibody
Question
Would A06685 anti-COL14A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
As indicated on the product datasheet, A06685 anti-COL14A1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-27
Question
We are currently using anti-COL14A1 antibody A06685 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The anti-COL14A1 antibody (A06685) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-28
Question
Is this A06685 anti-COL14A1 antibody reactive to the isotypes of COL14A1?
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
The immunogen of A06685 anti-COL14A1 antibody is Synthetic peptide from human protein at AA range: 1571-1620. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-11
Question
I have attached the WB image, lot number and protocol we used for descending thoracic aorta using anti-COL14A1 antibody A06685. Please let me know if you require anything else.
J. Patel
Verified customer
Asked: 2017-05-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-29
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for descending thoracic aorta using anti-COL14A1 antibody A06685. Let me know if you need anything else.
S. Walker
Verified customer
Asked: 2015-03-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-03-06
