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Product Info Summary
| SKU: | A10525 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Collagen alpha-3(V) chain COL5A3 Antibody
SKU/Catalog Number
A10525
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Collagen alpha-3 (V) chain COL5A3 Antibody catalog # A10525. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen alpha-3(V) chain COL5A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10525)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen V alpha3. AA range:221-270
Reactive Species
A10525 is reactive to COL5A3 in Human
Calculated molecular weight
172.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10525 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using COL5A3 Polyclonal Antibody
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Western Blot analysis of K562 cells using COL5A3 Polyclonal Antibody
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen V alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using Collagen V alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Collagen alpha-3(V) chain COL5A3 Antibody (A10525)
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Customer Q&As
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4 Customer Q&As for Anti-Collagen alpha-3(V) chain COL5A3 Antibody
Question
We are currently using anti-COL5A3 antibody A10525 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The anti-COL5A3 antibody (A10525) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-COL5A3 antibody A10525. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-02
Question
Do you have a BSA free version of anti-COL5A3 antibody A10525 available?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-COL5A3 antibody A10525 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-31
Question
I see that the anti-COL5A3 antibody A10525 works with WB, what is the protocol used to produce the result images on the product page?
K. Moore
Verified customer
Asked: 2017-10-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-10-05
