Product Info Summary
| SKU: | B02908 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Bovine, Human |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-Collagen Type IV COL4A4 Antibody Biotin Conjugated
View all COL4A1-COL4A6 Antibodies
SKU/Catalog Number
B02908
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-Collagen Type IV COL4A4 Antibody Biotin Conjugated (Catalog # B02908). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Bovine.
Storage & Handling
Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)
Cite This Product
Anti-Collagen Type IV COL4A4 Antibody Biotin Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # B02908)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
B02908 is reactive to COL4A1-COL4A6 in Bovine, Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
160.6 kDa
Background of COL4A1-COL4A6
Anti-Collagen Type IV detects collagen. Type-IV collagen is a type of collagen found primarily in the basal lamina. It is the major structural element of basal membranes. Anti-Collagen Type IV antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
B02908 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000 - 1:100,000
IHC: 1:1,000 - 1:5,000
IP: 1:100
WB: 1:2,000 - 1:10,000
Anti-Collagen Type IV is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Specific Publications For Anti-Collagen Type IV COL4A4 Antibody Biotin Conjugated (B02908)
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3 Customer Q&As for Anti-Collagen Type IV COL4A4 Antibody Biotin Conjugated
Question
I was wanting to use your anti-Collagen Type IV antibody Biotin Conjugated for IHC for human eye on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human eye identification?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
You can see on the product datasheet, B02908 anti-Collagen Type IV antibody Biotin Conjugated has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-Collagen Type IV antibody Biotin Conjugated B02908 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The anti-Collagen Type IV antibody Biotin Conjugated (B02908) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-31
Question
We need to test anti-Collagen Type IV antibody Biotin Conjugated B02908 on human eye for research purposes, then I may be interested in using anti-Collagen Type IV antibody Biotin Conjugated B02908 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The products we sell, including anti-Collagen Type IV antibody Biotin Conjugated B02908, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-23


