Anti-Collagen Type IV COL4A4 Antibody Biotin Conjugated

Product Name

Anti-Collagen Type IV COL4A4 Antibody Biotin Conjugated

View all Collagen IV alpha 1 Antibodies

SKU/Catalog Number

B02908

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Collagen Type IV COL4A4 Antibody Biotin Conjugated (Catalog # B02908). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Bovine.

Storage & Handling

Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Collagen Type IV COL4A4 Antibody Biotin Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # B02908)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

B02908 is reactive to COL4A1 in Bovine, Human

Applications

B02908 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

160.611kDa

Background of Collagen IV alpha 1

Anti-Collagen Type IV detects collagen. Type-IV collagen is a type of collagen found primarily in the basal lamina. It is the major structural element of basal membranes. Anti-Collagen Type IV antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IHC: 1:1,000 - 1:5,000
IP: 1:100
WB: 1:2,000 - 1:10,000

Validation Images & Assay Conditions

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Collagen IV was detected in paraffin-embedded sections of human kidney tissue (Left) and human liver tissue (Right) using rabbit anti-Collagen Type IV biotin conjugated polyclonal antibody (Catalog # B02908) at 10

Gene/Protein Information For COL4A1 (Source: Uniprot.org, NCBI)

Alternative Names

alpha-1 chain; Arresten; COL4A1 NC1 domain; COL4A1; collagen alpha-1(IV) chain; Collagen IV alpha 1; collagen IV, alpha-1 polypeptide; collagen, type IV, alpha 1; Collagen-4; EC 3.4.23; EC 6.3.1.2 COL4A1 BSVD, BSVD1s, PADMAL, RATOR, COL4A1 collagen type IV alpha 1 chain collagen alpha-1(IV) chain|COL4A1 NC1 domain|arresten|collagen IV, alpha-1 polypeptide|collagen of basement membrane, alpha-1 chain

For more info on COL4A1, check out the COL4A1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COL4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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