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GJA1 antibody (A00599) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 23 Publications

a00599 gja1 primary antibodies ihc testing 1

IHC analysis of GJA1 using anti-GJA1 antibody (A00599).
GJA1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (A00599) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-Connexin 43/GJA1 Antibody Picoband®

GJA1 antibody

23 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, IHC, WB

Tested Applications

GJA1 antibody was positively detected by WB in:

human 293T whole cell, human U251 whole cell, human A549 whole cell, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

GJA1 antibody was positively detected by IHC in:

human cervical cancer tissue, rat heart tissue, mouse heart tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Rabbit

Predicted

Dog

Predicted

Bovine

Predicted

Sheep

Predicted

Product Information

Product Name

Anti-Connexin 43/GJA1 Antibody Picoband®
View all GJA1 Antibodies

Form

Lyophilized

Uniprot

P17302

Gene ID

2697

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Connexin 43/GJA1 recombinant protein (Position: D3-R362).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

43 kDa

Calculated Molecular Weight

43.0 kDa

Background of GJA1

Connexin 43 (Cx43), also called GAP Junction Protein, alpha-1 (GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

Cite This Product

Anti-Connexin 43/GJA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00599)

Protocols

Western Blot protocol for GJA1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for GJA1 antibody

Review Protocol

Publications

Exploring the Role of Nesprin1 in isoproterenol-induced heart failure: Insights into pathogenesis and therapeutic implications

Published: 2026-08-11

Journal: PLoS One

Application: WB,IF

Impact Factor: 2.80

Species: Mouse

PMID: 42579703

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Anti-Connexin 43/GJA1 Antibody Picoband®

GJA1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Exploring the Role of Nesprin1 in isoproterenol-induced heart failure: Insights into pathogenesis and therapeutic implications

Cellular evidence and spatial distribution of endosomal biosynthesis and autophagy in intestinal immune barrier cells of crucian carp (Carassius carassius)

SARS-CoV-2 Structural Proteins Modulated Blood-Testis Barrier-Related Proteins through Autophagy in the Primary Sertoli Cells

Pinocembrin Inhibits P2X4 Receptor–Mediated Pyroptosis in Hippocampus to Alleviate the Behaviours of Chronic Pain and Depression Comorbidity in Rats

Remodelling 3D printed GelMA-HA corneal scaffolds by cornea stromal cells

PGC-1α activator ZLN005 promotes maturation of cardiomyocytes derived from human embryonic stem cells

Effect of celecoxib on protein expression of FAK and Cx43 in DMBA induced rat tongue carcinoma cells

Cx32 exerts anti-apoptotic and pro-tumor effects via the epidermal growth factor receptor pathway in hepatocellular carcinoma

Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway

Mitochondrial Uncoupling Protein 2 in human cumulus cells is associated with regulating autophagy and apoptosis, maintaining gap junction integrity and progesterone synthesis

Ectoderm mesenchymal stem cells promote differentiation and maturation of oligodendrocyte precursor cells

The Compound Chinese Medicine “Kang Fu Ling” Protects against High Power Microwave-Induced Myocardial Injury

Expression of Cx43 and Pax3 proteins in the human placental villi and decidua during early pregnancy

AQP4 KO exacerbating renal dysfunction is mediated by endoplasmic reticulum stress and p66Shc and is attenuated by apocynin and endothelin antagonist CPU0213

Apocynin and raisanberine alleviate intermittent hypoxia induced abnormal StAR and 3β-HSD and low testosterone by suppressing endoplasmic reticulum stress and activated p66Shc in rat testes

Recombinant human brain natriuretic peptide therapy combined with bone mesenchymal stem cell transplantation for treating heart failure in rats

Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats

Panax notoginseng Saponins Potentiate Osteogenesis of Bone Marrow Stromal Cells by Modulating Gap Junction Intercellular Communication Activities

The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol

Clinical significance of vascular endothelial growth factor and connexin43 for predicting pancreatic cancer clinicopathologic parameters

Differential expression of connexin 43 in human autoimmune thyroid disease

Chemical and physical stimuli induce cardiomyocyte differentiation from stem cells

Fibrosis of Collagen I and Remodeling of Connexin 43 in Atrial Myocardium of Patients with Atrial Fibrillation

Review

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