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Product Info Summary
| SKU: | A04843 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-COP1 RFWD2 Antibody
SKU/Catalog Number
A04843
Size
100μl
Form
Liquid
Description
Boster Bio Anti-COP1 RFWD2 Antibody catalog # A04843. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COP1 RFWD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04843)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
7C1
Isotype
IgG
Immunogen
Synthesized peptide derived from human COP1 around the non-phosphorylation site of S387.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04843 is reactive to COP1 in Human, Mouse
Applications
A04843 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
80.474kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 293T lysis using COP1 antibody.
Protein Target Info & Infographic
Gene/Protein Information For COP1 (Source: Uniprot.org, NCBI)
Gene Name
COP1
Full Name
E3 ubiquitin-protein ligase COP1
Weight
80.474kDa
Superfamily
COP1 family
Alternative Names
E3 ubiquitin-protein ligase COP1 COP1 CFAP78, FAP78, RFWD2, RNF200 COP1 E3 ubiquitin ligase E3 ubiquitin-protein ligase COP1|E3 ubiquitin-protein ligase RFWD2|RING finger protein 200|RING-type E3 ubiquitin transferase RFWD2|constitutive photomorphogenesis protein 1 homolog|constitutive photomorphogenic protein 1|putative ubiquitin ligase COP1|ring finger and WD repeat domain 2, E3 ubiquitin protein ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COP1, check out the COP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COP1 RFWD2 Antibody (A04843)
Hello CJ!
No publications found for A04843
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-COP1 RFWD2 Antibody
Question
Would A04843 anti-COP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
You can see on the product datasheet, A04843 anti-COP1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-16
Question
I have a question about product A04843, anti-COP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04843 anti-COP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-18
Question
I see that the anti-COP1 antibody A04843 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-26
Question
See below the WB image, lot number and protocol we used for testis {eco:0000313 using anti-COP1 antibody A04843. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-13
Question
Is a blocking peptide available for product anti-COP1 antibody (A04843)?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
We do provide the blocking peptide for product anti-COP1 antibody (A04843). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-13
Question
Would anti-COP1 antibody A04843 work on dog WB with testis {eco:0000313?
M. Li
Verified customer
Asked: 2019-08-19
Answer
Our lab technicians have not tested anti-COP1 antibody A04843 on dog. You can run a BLAST between dog and the immunogen sequence of anti-COP1 antibody A04843 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog testis {eco:0000313 in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-19
Question
We are interested in to test anti-COP1 antibody A04843 on mouse testis {eco:0000313 for research purposes, then I may be interested in using anti-COP1 antibody A04843 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Williams
Verified customer
Asked: 2019-03-11
Answer
The products we sell, including anti-COP1 antibody A04843, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-11
Question
Is this A04843 anti-COP1 antibody reactive to the isotypes of RFWD2?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
The immunogen of A04843 anti-COP1 antibody is Synthesized peptide derived from human COP1 around the non-phosphorylation site of S387. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-11
Question
Would anti-COP1 antibody A04843 work for WB with testis {eco:0000313?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
According to the expression profile of testis {eco:0000313, RFWD2 is highly expressed in testis {eco:0000313. So, it is likely that anti-COP1 antibody A04843 will work for WB with testis {eco:0000313.
Boster Scientific Support
Answered: 2018-01-31
Question
I was wanting to use your anti-COP1 antibody for WB for mouse testis eco:0000313 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been validated and is this antibody a good choice for mouse testis {eco:0000313 identification?
M. Mangal
Verified customer
Asked: 2015-04-06
Answer
You can see on the product datasheet, A04843 anti-COP1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis {eco:0000313 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-04-06
Question
Is there a BSA free version of anti-COP1 antibody A04843 available?
S. Parker
Verified customer
Asked: 2015-02-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-COP1 antibody A04843 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-02-10
Question
We are currently using anti-COP1 antibody A04843 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
J. Zhao
Verified customer
Asked: 2014-10-31
Answer
The anti-COP1 antibody (A04843) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis {eco:0000313 using anti-COP1 antibody A04843. Let me know if you need anything else.
J. Jones
Verified customer
Asked: 2013-11-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-11
