Anti-CRLF2 Antibody Picoband™

Product Name

Anti-CRLF2 Antibody Picoband™

View all TSLPR/CRLF2 Antibodies

SKU/Catalog Number

A04167-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CRLF2 Antibody Picoband™ catalog # A04167-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CRLF2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04167-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human CRLF2 recombinant protein (Position: A26-K231).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04167-1 is reactive to Crlf2 in Human

Applications

A04167-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

65-75 kDa

Calculated molecular weight

37.762kDa

Background of TSLPR/CRLF2

Cytokine receptor-like factor 2 is a protein that in humans is encoded by the CRLF2 gene. This gene encodes a member of the type I cytokine receptor family. The encoded protein is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Rearrangement of this gene with immunoglobulin heavy chain gene (IGH) on chromosome 14, or with P2Y purinoceptor 8 gene (P2RY8) on the same X or Y chromosomes is associated with B-progenitor acute lymphoblastic leukemia (ALL) and Down syndrome ALL. Alternatively spliced transcript variants have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CRLF2 using anti-CRLF2 antibody (A04167-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human CRLF2 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRLF2 antigen affinity purified polyclonal antibody (Catalog # A04167-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRLF2 at approximately 65-75KD. The expected band size for CRLF2 is at 51KD.

Gene/Protein Information For Crlf2 (Source: Uniprot.org, NCBI)

Alternative Names

CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR Crlf2|CRLM2, Ly11, Ly114, T, Tpte, Tpte2, Tslpr|cytokine receptor-like factor 2|cytokine receptor-like factor 2|TSLP receptor|cytokine receptor-like molecule 2|lymphocyte antigen 114|thymic stromal lymphopoietin protein receptor|thymic stromal-derived lymphopoietin, receptor|transmembrane phosphoinositide 3-phosphatase and tensin homolog 2|type I cytokine receptor delta 1

For more info on Crlf2, check out the Crlf2 Infographic

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In this infographic, you will see the following information for Crlf2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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