Anti-CTRP1 C1QTNF1 Antibody

Product Name

Anti-CTRP1 C1QTNF1 Antibody

View all C1QTNF1 Antibodies

SKU/Catalog Number

A09550-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CTRP1 C1QTNF1 Antibody (Catalog # A09550-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CTRP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CTRP1 C1QTNF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09550-1)

Host

Rabbit

Contents

CTRP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CTRP1 (NT) antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human CTRP1. The immunogen is located within amino acids 40 - 90 of CTRP1.

Cross-reactivity

These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size.

Reactive Species

A09550-1 is reactive to C1QTNF1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

31.7 kDa

Background of C1QTNF1

Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. In obese (ob/ob) mice, RT-PCR analysis showed that mCTRP1 transcripts are seen at substantially higher levels in adipose tissues compared to those of normal mice.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09550-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CTRP1 antibody can be used for the detection of CTRP1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information C1QTNF1

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