Anti-CXCL2 Antibody Picoband™

Product Name

Anti-CXCL2 Antibody Picoband™

View all CXCL2 Antibodies

SKU/Catalog Number

PB9858

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CXCL2 Antibody Picoband™ catalog # PB9858. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CXCL2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9858)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived rat CXCL2 recombinant protein (Position: S32-N100). Rat CXCL2 shares 62.3% and 88.4% amino acid (aa) sequence identity with huamn and mouse CXCL2, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9858 is reactive to CXCL2 in Mouse, Rat

Applications

PB9858 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

11 kDa

Calculated molecular weight

11.389kDa

Background of CXCL2

Chemokine (C-X-C motif) ligand 2  (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha  (MIP2-alpha), Growth-regulated protein beta  (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CXCL2 using anti-CXCL2 antibody (PB9858).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat CXCL2 protein 0.5ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL2 antigen affinity purified polyclonal antibody (Catalog # PB9858) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL2 at approximately 11 kDa. The expected band size for CXCL2 is at 11 kDa.

Gene/Protein Information For CXCL2 (Source: Uniprot.org, NCBI)

Alternative Names

chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2 CXCL2 CINC-2a, GRO2, GROb, MGSA-b, MIP-2a, MIP2, MIP2A, SCYB2 C-X-C motif chemokine ligand 2 C-X-C motif chemokine 2|GRO2 oncogene|MGSA beta|MIP2-alpha|chemokine (C-X-C motif) ligand 2|gro-beta|growth-regulated protein beta|macrophage inflammatory protein 2-alpha|melanoma growth stimulatory activity beta

For more info on CXCL2, check out the CXCL2 Infographic

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In this infographic, you will see the following information for CXCL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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