Anti-CXCR1 Antibody

Product Name

Anti-CXCR1 Antibody

View all CXCR1/IL-8RA Antibodies

SKU/Catalog Number

PA2080

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CXCR1 Antibody catalog # PA2080. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CXCR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2080)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2080 is reactive to CXCR1 in Human

Applications

PA2080 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

39.791kDa

Background of CXCR1/IL-8RA

CXCR1 (Chemokine,CXC Motif, Receptor 1), also known as IL8RA or IL8R1, is a chemokine receptor. IL8RA is its human gene. Morris et al. (1992) mapped the IL8RA gene to chromosome 2q35. Hartl et al. (2007) concluded that CXCR1 cleavage and its functional consequences represent an important pathophysiologic mechanism in CF and other neutrophilic airway diseases. Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CXCR1 using anti-CXCR1 antibody (PA2080).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR1 antigen affinity purified polyclonal antibody (Catalog # PA2080) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR1 at approximately 50 kDa. The expected band size for CXCR1 is at 40 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of human PBMC cells using anti-CXCR1 antibody (PA2080).
Overlay histogram showing human PBMC cells stained with PA2080 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR1 Antibody (PA2080, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CXCR1 (Source: Uniprot.org, NCBI)

Alternative Names

CD128; CD181 antigen; CD181; CDw128aC-C; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXCR1; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 RA; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL8RAC-C-CKR-1; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A CXCR1 C-C, C-C-CKR-1, CD128, CD181, CDw128a, CKR-1, CMKAR1, IL8R1, IL8RA, IL8RBA C-X-C motif chemokine receptor 1 C-X-C chemokine receptor type 1|CXC-R1|CXCR-1|IL-8 receptor type 1|IL-8R A|chemokine (C-X-C motif) receptor 1|high affinity interleukin-8 receptor A|interleukin 8 receptor, alpha|interleukin-8 receptor type 1|interleukin-8 receptor type A

For more info on CXCR1, check out the CXCR1 Infographic

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In this infographic, you will see the following information for CXCR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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