Anti-CXXC5 Antibody

Product Name

Anti-CXXC5 Antibody

View all CXXC5 Antibodies

SKU/Catalog Number

A06865

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CXXC5 Antibody (Catalog # A06865). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CXXC5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CXXC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06865)

Host

Rabbit

Contents

CXXC5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CXXC5 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CXXC5. The immunogen is located within amino acids 30 - 80 of CXXC5.

Cross-reactivity

At least two isoforms of CXXC5 are known to exist. CXXC5 antibody is predicted to not cross-react with other CXXC protein family members.

Reactive Species

A06865 is reactive to CXXC5 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

33.0 kDa

Background of CXXC5

CXXC5 is zinc finger protein closely related to the DVL1-binding protein CXXC4 (also known as Idax), and like CXXC4 is thought to modulate the Wnt signaling pathway during development. CXXC5 is upregulated in neural stem cells upon BMP4 stimulation, leading to decreased levels of the Wnt-signaling target AXIN2 and facilitated the response of the stem cells to Wnt3a. Recent reports suggest CXXC5 transcription is activated by the Wilms tumor 1 (WT1) transcription factor, and together are involved in the WNT/beta-catenin pathway and play a role in embryonic kidney development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06865 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CXXC5 antibody can be used for detection of CXXC5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CXXC5

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