Anti-Cybr CYTIP Antibody

Product Name

Anti-Cybr CYTIP Antibody

View all CYTIP Antibodies

SKU/Catalog Number

A09710-1

Size

25uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Cybr CYTIP Antibody (Catalog # A09710-1). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Expiration date is three (3) months from date of opening.

Cite This Product

Anti-Cybr CYTIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09710-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Clone Number

A12

Isotype

IgG

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the carboxy terminal portion of mouse Cybr.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09710-1 is reactive to CYTIP in Mouse

Applications

A09710-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

40.01kDa

Background of CYTIP

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK Macrophages, dendritic cells). Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
IHC: User optimized
WB: 1:1000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CYTIP using anti-CYTIP antibody (A09710-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYTIP antigen affinity purified polyclonal antibody (Catalog # A09710-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CYTIP.

Gene/Protein Information For CYTIP (Source: Uniprot.org, NCBI)

Alternative Names

B3-1; CASPCytohesin-associated scaffolding protein; Cbp HE; CYBRCytohesin-binding protein HE; CYTHIP; cytohesin 1 interacting protein; Cytohesin binder and regulator; cytohesin-1 interacting protein; cytohesin-interacting protein; HEPleckstrin homology Sec7 and coiled-coil domains-binding protein; pleckstrin homology, Sec7 and coiled/coil domains, binding protein; pleckstrin homology, Sec7 and coiled-coil domains, binding protein; PSCDBPcytohesin binding protein HE CYTIP B3-1, CASP, CYBR, CYTHIP, HE, PSCDBP cytohesin 1 interacting protein cytohesin-interacting protein|cbp HE|cytohesin binder and regulator|cytohesin binding protein HE|cytohesin-associated scaffolding protein|pleckstrin homology Sec7 and coiled-coil domains-binding protein

For more info on CYTIP, check out the CYTIP Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CYTIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the CYTIP Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis