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Product Info Summary
| SKU: | M00543 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00543
BM4652 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody catalog # M00543. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00543)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GHB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin E1
Reactive Species
M00543 is reactive to CCNE1 in Human
Observed Molecular Weight
47 kDa
Calculated molecular weight
47.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00543 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
Positive Control
ICC/IF: Hela cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of Cyclin E1 expression in HeLa cell lysate.
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Immunofluorescent analysis of Hela cells, using Cyclin E1 Antibody.
Specific Publications For Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody (M00543)
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Customer Q&As
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7 Customer Q&As for Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody
Question
Would anti-Cyclin E1 Rabbit Monoclonal antibody M00543 work for IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
According to the expression profile of cervix carcinoma, CCNE1 is highly expressed in cervix carcinoma. So, it is likely that anti-Cyclin E1 Rabbit Monoclonal antibody M00543 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2020-02-11
Question
Is a blocking peptide available for product anti-Cyclin E1 Rabbit Monoclonal antibody (M00543)?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
We do provide the blocking peptide for product anti-Cyclin E1 Rabbit Monoclonal antibody (M00543). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-17
Question
I am interested in to test anti-Cyclin E1 Rabbit Monoclonal antibody M00543 on human cervix carcinoma for research purposes, then I may be interested in using anti-Cyclin E1 Rabbit Monoclonal antibody M00543 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The products we sell, including anti-Cyclin E1 Rabbit Monoclonal antibody M00543, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-15
Question
I have a question about product M00543, anti-Cyclin E1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00543 anti-Cyclin E1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-19
Question
Will anti-Cyclin E1 Rabbit Monoclonal antibody M00543 work on dog WB with secondary oocyte?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
Our lab technicians have not tested anti-Cyclin E1 Rabbit Monoclonal antibody M00543 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Cyclin E1 Rabbit Monoclonal antibody M00543 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog secondary oocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-05
Question
Will M00543 anti-Cyclin E1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-14
Answer
It shows on the product datasheet, M00543 anti-Cyclin E1 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-14
Question
I was wanting to use your anti-Cyclin E1 Rabbit Monoclonal antibody for IF for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-08-31
Answer
As indicated on the product datasheet, M00543 anti-Cyclin E1 Rabbit Monoclonal antibody has been validated for IF, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-31
