Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody

Boster Bio Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody catalog # M00543. Tested in IF, ICC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M00543
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, ICC, WB

Product Name

Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody

See all Cyclin E1 products

SKU/Catalog Number

M00543

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody catalog # M00543. Tested in IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00543)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GHB-3

Immunogen

A synthesized peptide derived from human Cyclin E1

Reactive Species

M00543 is reactive to CCNE1 in Human

Applications

M00543 is guaranteed for IF, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For CCNE1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P24864

Gene ID

898

Gene Name

CCNE1

Full Name

G1/S-specific cyclin-E1

Weight

47.077kDa

Superfamily

cyclin family

Alternative Names

CCNE; CCNE1; CCNEcyclin Es; Cyclin E1; cyclin Et; G1/S-specific cyclin-E1 CCNE1 CCNE, pCCNE1 cyclin E1 G1/S-specific cyclin-E1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCNE1, check out the CCNE1 Infographic

CCNE1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCNE1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00543 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wu Y, Tao Y, Chen Y, Xu W. Plos One. 2012;7(11):E48917. Doi: 10.1371/Journal.Pone.0048917. Epub 2012 Nov 7. Rhoc Regulates The Proliferation Of Gastric Cancer Cells Through Interaction With Iqgap1.

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7 Customer Q&As for Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody

Question

Would anti-Cyclin E1 Rabbit Monoclonal antibody M00543 work for IF with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

According to the expression profile of cervix carcinoma, CCNE1 is highly expressed in cervix carcinoma. So, it is likely that anti-Cyclin E1 Rabbit Monoclonal antibody M00543 will work for IF with cervix carcinoma.

Boster Scientific Support

Answered: 2020-02-11

Question

Is a blocking peptide available for product anti-Cyclin E1 Rabbit Monoclonal antibody (M00543)?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

We do provide the blocking peptide for product anti-Cyclin E1 Rabbit Monoclonal antibody (M00543). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-17

Question

I am interested in to test anti-Cyclin E1 Rabbit Monoclonal antibody M00543 on human cervix carcinoma for research purposes, then I may be interested in using anti-Cyclin E1 Rabbit Monoclonal antibody M00543 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

The products we sell, including anti-Cyclin E1 Rabbit Monoclonal antibody M00543, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-15

Question

I have a question about product M00543, anti-Cyclin E1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00543 anti-Cyclin E1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-19

Question

Will anti-Cyclin E1 Rabbit Monoclonal antibody M00543 work on dog WB with secondary oocyte?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

Our lab technicians have not tested anti-Cyclin E1 Rabbit Monoclonal antibody M00543 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Cyclin E1 Rabbit Monoclonal antibody M00543 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog secondary oocyte in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-05

Question

Will M00543 anti-Cyclin E1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-05-14

Answer

It shows on the product datasheet, M00543 anti-Cyclin E1 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-05-14

Question

I was wanting to use your anti-Cyclin E1 Rabbit Monoclonal antibody for IF for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2017-08-31

Answer

As indicated on the product datasheet, M00543 anti-Cyclin E1 Rabbit Monoclonal antibody has been validated for IF, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-31

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M00543
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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