Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody

Product Name

Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody

View all Cyclin E1 Antibodies

SKU/Catalog Number

M00543

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody catalog # M00543. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin E1 CCNE1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00543)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GHB-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Cyclin E1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00543 is reactive to CCNE1 in Human

Applications

M00543 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

47.077kDa

Background of Cyclin E1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

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Western blot analysis of Cyclin E1 expression in HeLa cell lysate.

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Immunofluorescent analysis of Hela cells, using Cyclin E1 Antibody.

Gene/Protein Information For CCNE1 (Source: Uniprot.org, NCBI)

Alternative Names

CCNE; CCNE1; CCNEcyclin Es; Cyclin E1; cyclin Et; G1/S-specific cyclin-E1 CCNE1 CCNE, pCCNE1 cyclin E1 G1/S-specific cyclin-E1

For more info on CCNE1, check out the CCNE1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCNE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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