Anti-CYFIP1 Rabbit Monoclonal Antibody

Product Name

Anti-CYFIP1 Rabbit Monoclonal Antibody

View all Cyfip1 Antibodies

SKU/Catalog Number

M04596

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-CYFIP1 Rabbit Monoclonal Antibody catalog # M04596. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CYFIP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04596)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

29C25

Isotype

IgG

Immunogen

A synthesized peptide derived from human CYFIP1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04596 is reactive to Cyfip1 in Human, Mouse, Rat

Applications

M04596 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

145 kDa

Calculated molecular weight

145.241kDa

Background of Cyfip1

Putative transcription factor involved in pancreas development and function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CYFIP1 using anti-CYFIP1 antibody (M04596).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYFIP1 antigen affinity purified monoclonal antibody (Catalog # M04596) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYFIP1 at approximately 145 kDa. The expected band size for CYFIP1 is at 145 kDa.

Gene/Protein Information For Cyfip1 (Source: Uniprot.org, NCBI)

Alternative Names

cytoplasmic FMR1 interacting protein 1; cytoplasmic FMR1-interacting protein 1; FLJ45151; KIAA0068cytoplasmic FMRP interacting protein 1; P140SRA-1; selective hybridizing clone; SHYC; Specifically Rac1-associated protein 1; SRA1; sra-1 Cyfip1|E030028J09Rik, P140S, P140SRA-1, P140sra1, Shy, Shyc, Sr, Sra-1, Sra1, l(7), l(7)1Rl, l7, l71Rl, l7Rl1, mKIAA0068, pl, pl-1|cytoplasmic FMR1 interacting protein 1|cytoplasmic FMR1-interacting protein 1|specifically Rac1-associated protein 1

For more info on Cyfip1, check out the Cyfip1 Infographic

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In this infographic, you will see the following information for Cyfip1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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