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Product Info Summary
| SKU: | A02121-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CYP27A1 Antibody Picoband®
SKU/Catalog Number
A02121-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CYP27A1 Antibody Picoband® catalog # A02121-2. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP27A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02121-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CYP27A1 recombinant protein (Position: A7-C531).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02121-2 is reactive to CYP27A1 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
60.2 kDa
Background of CYP27A1
CYP27A1 is a gene encoding a cytochrome P450 oxidase, and is commonly known as sterol 27-hydroxylase. It is mapped to 2q35. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02121-2 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human placenta tissue, human HEPG2 whole cell, human CACO-2 whole cell, human SW620 whole cell, human SGC-7901 whole cell, human HEK293 whole cell, monkey liver tissue, human U20S whole cell, rat liver tissue, rat heart tissue, mouse liver tissue, mouse heart tissue, mouse NIH/3T3 whole cell
IHC: human liver tissue
ICC/IF: A431 cell
Validation Images & Assay Conditions
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IF analysis of CYP27A1 using anti-CYP27A1 antibody (A02121-2).
CYP27A1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-CYP27A1 Antibody (A02121-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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IHC analysis of CYP27A1 using anti-CYP27A1 antibody (A02121-2).
CYP27A1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP27A1 Antibody (A02121-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Western blot analysis of CYP27A1 using anti-CYP27A1 antibody (A02121-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HEPG2 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: human SGC-7901 whole cell lysates,
Lane 6: human HEK293 whole cell lysates,
Lane 7: monkey liver tissue lysates,
Lane 8: human U20S whole cell lysates,
Lane 9: rat liver tissue lysates,
Lane 10: rat heart tissue lysates,
Lane 11: mouse liver tissue lysates,
Lane 12: mouse heart tissue lysates,
Lane 13: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP27A1 antigen affinity purified polyclonal antibody (Catalog # A02121-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP27A1 at approximately 60KD. The expected band size for CYP27A1 is at 60KD.
Specific Publications For Anti-CYP27A1 Antibody Picoband® (A02121-2)
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