Anti-CYP27A1 Antibody Picoband™

CYP27A1 antibody

Boster Bio Anti-CYP27A1 Antibody Picoband™ catalog # A02121-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Product Info Summary

SKU: A02121-2
Size: 100 μg/vial
Reactive Species: Human, Monkey, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, ICC, WB

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Product Name

Anti-CYP27A1 Antibody Picoband™

View all CYP27A1 Antibodies

SKU/Catalog Number

A02121-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CYP27A1 Antibody Picoband™ catalog # A02121-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CYP27A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02121-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CYP27A1 recombinant protein (Position: A7-C531).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02121-2 is reactive to CYP27A1 in Human, Monkey, Mouse, Rat

Applications

A02121-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

60.235kDa

Background of CYP27A1

CYP27A1 is a gene encoding a cytochrome P450 oxidase, and is commonly known as sterol 27-hydroxylase. It is mapped to 2q35. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse, Monkey, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CYP27A1 (Source: Uniprot.org, NCBI)

Gene Name

CYP27A1

Full Name

Sterol 26-hydroxylase, mitochondrial

Weight

60.235kDa

Superfamily

cytochrome P450 family

Alternative Names

12-alpha-triol 27-hydroxylase; 7-alpha; cholestanetriol 26-monooxygenase; CP27,5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase; CTX5-beta-cholestane-3-alpha; CYP275-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase; Cytochrome P450 27; cytochrome P450, family 27, subfamily A, polypeptide 1; cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinousxanthomatosis), polypeptide 1; Cytochrome P-450C27/25; EC 1.14.13.15; sterol 26-hydroxylase, mitochondrial; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase CYP27A1 CP27, CTX, CYP27 cytochrome P450 family 27 subfamily A member 1 sterol 26-hydroxylase, mitochondrial|5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase|5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase|cholestanetriol 26-monooxygenase|cytochrome P-450C27/25|cytochrome P450 27|cytochrome P450, family 27, subfamily A, polypeptide 1|cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1|sterol 27-hydroxylase|vitamin D(3) 25-hydroxylase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CYP27A1, check out the CYP27A1 Infographic

CYP27A1 infographic

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In this infographic, you will see the following information for CYP27A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A02121-2

100μg

$370
A02121-2-10ug

10μg sample (liquid)

$99
A02121-2-Biotin

100 μg Biotin conjugated

$570
A02121-2-Cy3

100 μg Cy3 conjugated

$570
A02121-2-Dylight488

100 μg Dylight488 conjugated

$570
A02121-2-Dylight550

100 μg Dylight550 conjugated

$570
A02121-2-Dylight594

100 μg Dylight594 conjugated

$570
A02121-2-FITC

100 μg FITC conjugated

$570
A02121-2-HRP

100 μg HRP conjugated

$570
A02121-2-APC

100 μg APC conjugated

$670
A02121-2-PE

100 μg PE conjugated

$670
A02121-2-carrier-free

Carrier Free

$370

More conjugates/formats

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A02121-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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