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- Table of Contents
CYP27A1 Antibodies
AND CYP27A1 ELISA kits, CYP27A1 Proteins
Many biological assays use antibodies to detect CYP27A1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with CYP27A1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop CYP27A1 antibodies...
Designing a Western blot for CYP27A1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CYP27A1 Western Blot GuideDesigning an IHC experiment for CYP27A1? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the CYP27A1 IHC GuideCYP27A1 Infographic by Boster Bio
All CYP27A1 Products
Product
Figures
Specificity
Applications
Price
Human, Monkey, Mouse, Rat
ELISA, IF, IHC, ICC, WB
$370
Cat # A02121-2
100 μg/vial
Cat # A02121-2
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # M02121-1
100 μl/vial
Cat # M02121-1
100 μl/vial
Human
ELISA
$480
Cat # EKC1909
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1909
1 kit, containing one 96-well plate and all necessary reagents
CYP27A1 Overview
Facts about Sterol 26-hydroxylase, mitochondrial.
Sterol 26-hydroxylase, mitochondrial (CYP27A1)
Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity.
.
Gene Information
| Human | |
|---|---|
| Gene Name: | CYP27A1 |
| Uniprot: | Q02318 |
| Entrez: | 1593 |
Family
| Belongs to: |
|---|
| cytochrome P450 family |
Alternative Names
12-alpha-triol 27-hydroxylase; 7-alpha; cholestanetriol 26-monooxygenase; CP27,5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase; CTX5-beta-cholestane-3-alpha; CYP275-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase; Cytochrome P450 27; cytochrome P450, family 27, subfamily A, polypeptide 1; cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinousxanthomatosis), polypeptide 1; Cytochrome P-450C27/25; EC 1.14.13.15; sterol 26-hydroxylase, mitochondrial; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase
Molecular Weight
Mass (kDA):
60.235 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 2q35 |
| Sequence: | 2; NC_000002.12 (218781733..218815293) |
Subcellular Localizations
Mitochondrion inner membrane; Peripheral membrane protein. Post-translationally targeted to mitochondria. All three of the receptor proteins in the TOM complex, TOMM70, TOMM20 and TOMM22 are required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein.
Diseases
- Xanthomatosis, Cerebrotendinous
- Xanthomatosis
- Atherosclerosis
- Xanthoma
- Malignant Neoplasms
- Vitamin D Deficiency
- Arteriosclerosis
- Neoplasms
- Prostatic Neoplasms
- Malignant Neoplasm Of Prostate
- Rickets
- Diarrhea
- Nervousness
Pathways
- Cholesterol Homeostasis
- Transport
- Excretion
- Cholesterol Efflux
- Secretion
- Cholesterol Transport
- Pathogenesis
- Lipid Storage
- Reverse Cholesterol Transport
- Translation
- Localization
- Cell Proliferation
- Regulation Of Cholesterol Homeostasis
PTMs
- Hydroxylation
- Oxidation
- Cleavage
- Acetylation
Research Areas
- Stem Cell Markers
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CYP27A1
bosterbio.com/bosterbio-gene-info-cards/CYP27A1
References
PMID: 1708392 by Cali J.J., et al. Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.
PMID: 7690968 by Guo Y.-D., et al. Transfected human liver cytochrome P-450 hydroxylates vitamin D analogs at different side-chain positions.