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Product Info Summary
| SKU: | A03557 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody
SKU/Catalog Number
A03557
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody catalog # A03557. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03557)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human CYP2R1. at AA range: 220-300
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03557 is reactive to CYP2R1 in Human, Monkey, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
57359 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03557 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Positive Control
Validation Images & Assay Conditions
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Western blot analysis of HT-29 cells using CYP2R1 Polyclonal Antibody
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Western blot analysis of lysates from HT29 cells, using Cytochrome P450 2R1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HT-29 cells using Cytochrome P450 2R1 antibody.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Cytochrome P450 2R1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CYP2R1 (Source: Uniprot.org, NCBI)
Gene Name
CYP2R1
Full Name
Vitamin D 25-hydroxylase
Weight
57359 MW
Superfamily
cytochrome P450 family
Alternative Names
Cytochrome P450 2R1; cytochrome P450, family 2, R1; cytochrome P450, family 2, subfamily R, polypeptide 1; EC 1.14.13.15; MGC4663; vitamin D 25-hydroxylase CYP2R1 cytochrome P450 family 2 subfamily R member 1 vitamin D 25-hydroxylase|cytochrome P450 2R1|cytochrome P450, family 2, R1|cytochrome P450, family 2, subfamily R, polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP2R1, check out the CYP2R1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP2R1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody (A03557)
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No publications found for A03557
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Customer Q&As
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6 Customer Q&As for Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody
Question
We are currently using anti-CYP2R1 antibody A03557 for monkey tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it likely that the antibody can work on feline tissues as well?
G. Krishna
Verified customer
Asked: 2020-02-11
Answer
The anti-CYP2R1 antibody (A03557) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-11
Question
Can you help my question with product A03557, anti-CYP2R1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03557 anti-CYP2R1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-26
Question
Is this A03557 anti-CYP2R1 antibody reactive to the isotypes of CYP2R1?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The immunogen of A03557 anti-CYP2R1 antibody is Synthesized peptide derived from the Internal region of human CYP2R1. at AA rangle: 220-300. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-03
Question
Here is the WB image, lot number and protocol we used for sperm using anti-CYP2R1 antibody A03557. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-05
Question
Is a blocking peptide available for product anti-CYP2R1 antibody (A03557)?
E. Kulkarni
Verified customer
Asked: 2017-02-15
Answer
We do provide the blocking peptide for product anti-CYP2R1 antibody (A03557). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-02-15
Question
Will anti-CYP2R1 antibody A03557 work for WB with sperm?
W. Jones
Verified customer
Asked: 2016-02-19
Answer
According to the expression profile of sperm, CYP2R1 is highly expressed in sperm. So, it is likely that anti-CYP2R1 antibody A03557 will work for WB with sperm.
Boster Scientific Support
Answered: 2016-02-19
