Vitamin D 25-hydroxylase Antibodies

AND CYP2R1 ELISA kits, Vitamin D 25-hydroxylase Proteins

Many biological assays use antibodies to detect Vitamin D 25-hydroxylase, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Vitamin D 25-hydroxylase in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Vitamin D 25-hydroxylase antibodies...

We validate the specificity of these antibodies to Vitamin D 25-hydroxylase by testing them on tissues known to express CYP2R1 positively and negatively. Browse below to find the CYP2R1 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for Vitamin D 25-hydroxylase? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the CYP2R1 IHC Guide

CYP2R1 Infographic by Boster Bio

All CYP2R1 Products

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Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody

Western Blot analysis of HT-29 cells using CYP2R1 Polyclonal Antibody

Western blot analysis of lysates from HT29 cells, using Cytochrome P450 2R1 Antibody

Western blot analysis of the lysates from HT-29 cells using Cytochrome P450 2R1 antibody

Human, Monkey, Mouse

ELISA, IF, IHC, WB

$399
Cat # A03557
100μl

Cytochrome P450 2R1 Colorimetric Cell-Based ELISA

Western blot analysis of extracts from HT29 cells

Human, Mouse

ELISA

$480
Cat # EKC1915
1 kit, containing one 96-well plate and all necessary reagents

CYP2R1 (NM_024514) Human Over-expression Lysate

Human

$628
Cat # LS120227
20 µg, 100 µg

CYP2R1 (NM_024514) Human Recombinant Protein

Coomassie blue staining of purified CYP2R1 protein

$1249
Cat # PROTQ6VVX0
20 µg

CYP2R1 Overview

Facts about Vitamin D 25-hydroxylase.

CYP2R1 3D structure. Source: alphafold

Vitamin D 25-hydroxylase (CYP2R1)

Has a D-25-hydroxylase action on the two forms of vitamin D, vitamin D(2) and D(3). .

Gene Information

Human
Gene Name:	CYP2R1
Uniprot:	Q6VVX0
Entrez:	120227

Family

Belongs to:

cytochrome P450 family

Alternative Names

Cytochrome P450 2R1; cytochrome P450, family 2, R1; cytochrome P450, family 2, subfamily R, polypeptide 1; EC 1.14.13.15; MGC4663; vitamin D 25-hydroxylase

Molecular Weight

Mass (kDA):
57.359 kDA

Genomic Context


Human

Location:	11p15.2
Sequence:	11; NC_000011.10 (14877440..14898915, complement)

Subcellular Localizations

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Diseases

Vitamin D Deficiency
Malignant Neoplasms
Rickets
Diabetes Mellitus
Asthma
Sclerosis
Diabetes Mellitus, Insulin-dependent
Multiple Sclerosis
Prostatic Neoplasms
Malignant Neoplasm Of Prostate
Fibrosis
Osteoporosis
Dysequilibrium Syndrome

Hepatitis
Liver Diseases
Hepatitis, Chronic
Vitamin Deficiency
Hepatitis C, Chronic
Hepatitis C
Autoimmune Reaction

Pathways

Pathogenesis
Transport
Cell Proliferation
Lactation
Aging
Immune Response
Response To Vitamin
Ossification
Spermatogenesis
Response To Vitamin D

Inflammatory Response
Sensitization
Cell Growth
Cognition

PTMs

Hydroxylation

Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CYP2R1

References

PMID: 12867411 by Cheng J.B., et al. De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25- hydroxylase.

PMID: 15465040 by Shinkyo R., et al. Metabolism of vitamin D by human microsomal CYP2R1.