Product Info Summary
SKU: | A00339-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-CYP3A4/5 (F386) Antibody
SKU/Catalog Number
A00339-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CYP3A4/5 (F386) Antibody catalog # A00339-1. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP3A4/5 (F386) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00339-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human Cytochrome P450 3A4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00339-1 is reactive to CYP3A4 in Human, Mouse, Rat
Applications
A00339-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
57.343kDa
Background of CYP3A4
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide.
Vuoristo M.M., J. Biol. Chem. 270:22873-22881(1995).
Mungall A.J., Nature 425:805-811(2003).
Lui V.C., Genomics 32:401-412(1996).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of CYP3A4 using Anti-CYP3A4/5 (F386) Antibody (A00339-1).
Immunohistochemistry (IHC) analyzes of CYP3A4/5 (F386) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibodyClick image to see more details
Figure 1. Western blotting validation for Anti-CYP3A4/5 (F386) Antibody A00339-1
Western blot (WB) analysis of CYP3A4/5 (F386) pAb at 1:500 dilutionLane1:HepG2 whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:The Liver tissue lysate of Rat(40ug)
Lane4:The Liver tissue lysate of Mouse(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CYP3A4 (Source: Uniprot.org, NCBI)
Gene Name
CYP3A4
Full Name
Cytochrome P450 3A4
Weight
57.343kDa
Superfamily
cytochrome P450 family
Alternative Names
Albendazole Monooxygenase; Albendazole Sulfoxidase; CP33; CP34; CYP3A; CYP3A3; CYP3A4; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; cytochrome P450 3A4; Cytochrome P450 HLp; Cytochrome P450 NF-25; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; Cytochrome P450-PCN1; EC 1.14.13.32; EC 1.14.13.67; EC 1.14.13.97; EC 1.14.14.1; glucocorticoid-inducible P450; HLP; MGC126680; NF-25; Nifedipine oxidase; P450C3; P450-III, steroid inducible; P450PCN1; Quinine 3- CYP3A4 CP33, CP34, CYP3A, CYP3A3, CYPIIIA3, CYPIIIA4, HLP, NF-25, P450C3, P450PCN1, VDDR3 cytochrome P450 family 3 subfamily A member 4 cytochrome P450 3A4|1,4-cineole 2-exo-monooxygenase|1,8-cineole 2-exo-monooxygenase|P450-III, steroid inducible|albendazole monooxygenase (sulfoxide-forming)|albendazole sulfoxidase|cholesterol 25-hydroxylase|cytochrome P450 3A3|cytochrome P450 HLp|cytochrome P450 NF-25|cytochrome P450, family 3, subfamily A, polypeptide 4|cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3|cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4|cytochrome P450-PCN1|glucocorticoid-inducible P450|nifedipine oxidase|quinine 3-monooxygenase|taurochenodeoxycholate 6-alpha-hydroxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP3A4, check out the CYP3A4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP3A4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP3A4/5 (F386) Antibody (A00339-1)
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No publications found for A00339-1
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