Click image to see more details
Product Info Summary
| SKU: | A02885 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cysteine protease ATG4B ATG4B Antibody
SKU/Catalog Number
A02885
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Cysteine protease ATG4B ATG4B Antibody (Catalog # A02885). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG4B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Cysteine protease ATG4B ATG4B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02885)
Host
Rabbit
Contents
ATG4B antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B. The immunogen is located within the last 50 amino acids of ATG4B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ATG4B antibody is human, mouse and rat reactive. At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.
Reactive Species
A02885 is reactive to ATG4B in Human, Mouse, Rat
Applications
A02885 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
44.294kDa
Background of ATG4B
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4B, also known as AUTL1, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4). ATG4B has been found to be a novel protective protein in inflammatory colitis (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG4B antibody can be used for detection of ATG4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 μg/mL.
Click image to see more details
Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 μg/ml.
Click image to see more details
Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For ATG4B (Source: Uniprot.org, NCBI)
Gene Name
ATG4B
Full Name
Cysteine protease ATG4B
Weight
44.294kDa
Superfamily
peptidase C54 family
Alternative Names
APG4 autophagy 4 homolog B (S. cerevisiae); APG4 autophagy 4 homolog B; Apg4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; AUTL1; AUTL1MGC1353; AUT-like 1 cysteine endopeptidase; autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; cysteine protease ATG4B; DKFZp586D1822; EC 3.4.22; EC 3.4.22.-; hAPG4B; KIAA0943 ATG4B APG4B, AUTL1 autophagy related 4B cysteine peptidase cysteine protease ATG4B|APG4 autophagy 4 homolog B|ATG4 autophagy related 4 homolog B|AUT-like 1 cysteine endopeptidase|autophagin-1|autophagy-related cysteine endopeptidase 1|autophagy-related protein 4 homolog B|hAPG4B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG4B, check out the ATG4B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG4B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cysteine protease ATG4B ATG4B Antibody (A02885)
Hello CJ!
No publications found for A02885
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cysteine protease ATG4B ATG4B Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Cysteine protease ATG4B ATG4B Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
