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Product Info Summary
| SKU: | A10507-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Cytochrome P450 2A7 CYP2A7 Antibody
SKU/Catalog Number
A10507-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 2A7 CYP2A7 Antibody catalog # A10507-1. Tested in WB,IHC,ICC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 2A7 CYP2A7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10507-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human RECS1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10507-1 is reactive to CYP2A7 in Human, Mouse, Rat
Applications
A10507-1 is guaranteed for IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
56.425kDa
Background of CYP2A7
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32, 22q12-q13.1) for this gene have been described.
Lai C.-H., Genome Res. 10:703-713(2000).
Suzuki T., J. Biol. Chem. 276:33181-33195(2001).
Kenmochi N., Genomics 77:65-70(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-Cytochrome P450 2A7 CYP2A7 Antibody A10507-1
Western blot (WB) analysis of CYP2A7 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysate
Lane2:sp2/0 cell lysate
Lane3:PC12 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CYP2A7 (Source: Uniprot.org, NCBI)
Gene Name
CYP2A7
Full Name
Cytochrome P450 2A7
Weight
56.425kDa
Superfamily
cytochrome P450 family
Alternative Names
CPA7; CPAD; CYP2A; CYPIIA7; cytochrome P450 2A7; Cytochrome P450 IIA4; cytochrome P450, family 2, subfamily A, polypeptide 7; cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 7; EC 1.14.14.1; P450-IIA4 CYP2A7 CPA7, CPAD, CYP2A, CYPIIA7, P450-IIA4 cytochrome P450 family 2 subfamily A member 7 cytochrome P450 2A7|cytochrome P450 IIA4|cytochrome P450, family 2, subfamily A, polypeptide 7|cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 7|cytochrome P450IIA4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP2A7, check out the CYP2A7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP2A7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 2A7 CYP2A7 Antibody (A10507-1)
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No publications found for A10507-1
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