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Product Info Summary
| SKU: | M00498 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytochrome P450 2D6 CYP2D6 Monoclonal Antibody
SKU/Catalog Number
M00498
BM5370 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 2D6 CYP2D6 Monoclonal Antibody catalog # M00498. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 2D6 CYP2D6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00498)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADBO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytochrome P450 2D6 Cytochrome P450 2D6 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.
Reactive Species
M00498 is reactive to CYP2D6 in Human
Observed Molecular Weight
50, 55, 80 kDa
Calculated molecular weight
55.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00498 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Cytochrome P450 2D6 expression in K562 cell lysate.
Specific Publications For Anti-Cytochrome P450 2D6 CYP2D6 Monoclonal Antibody (M00498)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Cytochrome P450 2D6 CYP2D6 Monoclonal Antibody
Question
My question regarding product M00498, anti-Cytochrome P450 2D6 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Singh
Verified customer
Asked: 2020-03-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00498 anti-Cytochrome P450 2D6 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-03
Question
See below the WB image, lot number and protocol we used for liver using anti-Cytochrome P450 2D6 Monoclonal antibody M00498. Please let me know if you require anything else.
J. Collins
Verified customer
Asked: 2017-12-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-22
Question
Does anti-Cytochrome P450 2D6 Monoclonal antibody M00498 work on horse IF with liver?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
Our lab technicians have not tested anti-Cytochrome P450 2D6 Monoclonal antibody M00498 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Cytochrome P450 2D6 Monoclonal antibody M00498 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse liver in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-27
Question
Is this M00498 anti-Cytochrome P450 2D6 Monoclonal antibody reactive to the isotypes of CYP2D6?
S. Edwards
Verified customer
Asked: 2015-02-16
Answer
The immunogen of M00498 anti-Cytochrome P450 2D6 Monoclonal antibody is A synthesized peptide derived from human Cytochrome P450 2D6 Cytochrome P450 2D6 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-16
