Product Info Summary
SKU: | M02305-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02305-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody catalog # M02305-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02305-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EEF-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 10
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02305-1 is reactive to KRT10 in Human, Mouse, Rat
Applications
M02305-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
22 kDa
Calculated molecular weight
58.827kDa
Background of KRT10
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Immunofluorescent analysis of HACAT cells, using Cytokeratin 10 Antibody.
Click image to see more details
Western blot analysis of Cytokeratin 10 expression in A431 cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human bladder, using Cytokeratin 10 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For KRT10 (Source: Uniprot.org, NCBI)
Gene Name
KRT10
Full Name
Keratin, type I cytoskeletal 10
Weight
58.827kDa
Superfamily
intermediate filament family
Alternative Names
BCIE; BIE; CK10; CK-10; cytokeratin 10; Cytokeratin-10; EHK; K10keratosis palmaris et plantaris; Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10; keratin, type I cytoskeletal 10; keratin-10; KPP KRT10 BCIE, BIE, CK10, EHK, K10, KPP keratin 10 keratin, type I cytoskeletal 10|CK-10|cytokeratin 10|keratin 10, type I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT10, check out the KRT10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody (M02305-1)
Hello CJ!
M02305-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Localization of Human Mesenchymal Stem Cells from Umbilical Cord Blood and Their Role in Repair of Diabetic Foot Ulcers in Rats
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Cytokeratin 10 KRT10 Rabbit Monoclonal Antibody
Question
We are currently using anti-Cytokeratin 10 Rabbit Monoclonal antibody M02305-1 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
The anti-Cytokeratin 10 Rabbit Monoclonal antibody (M02305-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-05