Anti-Cytokeratin 18 (KRT18) Monoclonal Antibody

Product Name

Anti-Cytokeratin 18 (KRT18) Monoclonal Antibody

View all KRT18 Antibodies

SKU/Catalog Number

M01357

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Cytokeratin 18 (KRT18) Monoclonal Antibody (Catalog # M01357). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Hamster, Ferret, Cow, Pig, Cat, Dog, Sheep, Goat, Horse, Marmoset monkey.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Cytokeratin 18 (KRT18) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01357)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: C-04; same as Ks18.04

Isotype

IgG1, kappa

Immunogen

Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01357 is reactive to KRT18 in Cow, Dog, Ferret, Goat, Hamster, Human, Mouse, Pig, Rat, Sheep, Horse, Cat, Marmoset monkey

Applications

M01357 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

48.058kDa

Background of KRT18

This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Anti-Cytokeratin 18 Mouse Monoclonal Antibody (C-04).

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Western Blot analysis of human A) intestine and B) stomach lysate using Anti-Cytokeratin 18 Mouse Monoclonal Antibody (C-04).

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Western Blot analysis of HCT116 cell lysate using Anti-Cytokeratin 18 Mouse Monoclonal Antibody (C-04).

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Flow Cytometric analysis of PFA-fixed HeLa cells using Anti-Cytokeratin 18 Mouse Monoclonal Antibody (C-04) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Gene/Protein Information For KRT18 (Source: Uniprot.org, NCBI)

Alternative Names

Cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; CK-18; CYK18; Cytokeratin 18; cytokeratin-18; K18; Keratin 18; keratin, type I cytoskeletal 18; keratin-18; KRT18 KRT18 CK-18, CYK18, K18 keratin 18 keratin, type I cytoskeletal 18|cell proliferation-inducing gene 46 protein|cytokeratin 18|keratin 18, type I

For more info on KRT18, check out the KRT18 Infographic

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In this infographic, you will see the following information for KRT18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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