Click image to see more details
Product Info Summary
| SKU: | M02101-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 19 KRT19 Rabbit Monoclonal Antibody
View all Cytokeratin 19 Antibodies
SKU/Catalog Number
M02101-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 19 KRT19 Rabbit Monoclonal Antibody catalog # M02101-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 19 KRT19 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02101-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CCO-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 19
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02101-1 is reactive to KRT19 in Human, Mouse
Applications
M02101-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
44.106kDa
Background of Cytokeratin 19
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of Cytokeratin 19 expression in HepG2 cell lysate.
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Immunohistochemical analysis of paraffin-embedded mouse colon, using Cytokeratin 19 Antibody .
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Immunofluorescent analysis of MCF-7 cells, using Cytokeratin 19 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For KRT19 (Source: Uniprot.org, NCBI)
Gene Name
KRT19
Full Name
Keratin, type I cytoskeletal 19
Weight
44.106kDa
Superfamily
intermediate filament family
Alternative Names
40-kDa keratin intermediate filament; CK19; CK-19; Cytokeratin 19; EndoC; K19; K1CS; keratin 19; keratin, type I cytoskeletal 19; keratin, type I, 40-kd; keratin-19; Krt19; MGC15366 KRT19 CK19, K19, K1CS keratin 19 keratin, type I cytoskeletal 19|40-kDa keratin intermediate filament|CK-19|cytokeratin 19|keratin 19, type I|keratin, type I, 40-kd
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT19, check out the KRT19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 19 KRT19 Rabbit Monoclonal Antibody (M02101-1)
Hello CJ!
M02101-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lu J, Gu Yp, Xu X, Liu Ml, Xie P, Song Hp. World J Gastroenterol. 2005 Jun 14;11(22):3426-30. Adult Islets Cultured In Collagen Gel Transdifferentiate Into Duct-Like Cells.
Li M, Zhang B, Zhang Z, Liu X, Qi X, Zhao J, Jiang Y, Zhai H, Ji Y, Luo D. Biomed Res Int. 2014;2014:981261. Doi: 10.1155/2014/981261. Epub 2014 May 22. Stem Cell-Like Circulating Tumor Cells Indicate Poor Prognosis In Gastric Cancer.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Cytokeratin 19 KRT19 Rabbit Monoclonal Antibody
Question
Can you confirm that recombinant technology has not been used in the production of M02101-1?
Verified customer
Asked: 2020-10-28
Answer
The Anti-Cytokeratin 19 KRT19 Rabbit Monoclonal Antibody (M02101-1) is a recombinant antibody. Recombinant antibodies are produced in vitro by cloning antibody genes for immune-specific heavy and light antibody chains into high-yield expression vectors. These vectors are then introduced into expression hosts (eg bacteria, yeast, or mammalian) to generate the recombinant monoclonal antibodies.
Boster Scientific Support
Answered: 2020-10-28
Question
I was wanting to use to test anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The products we sell, including anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-28
Question
Will M02101-1 anti-Cytokeratin 19 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
As indicated on the product datasheet, M02101-1 anti-Cytokeratin 19 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-19
Question
Is a blocking peptide available for product anti-Cytokeratin 19 Rabbit Monoclonal antibody (M02101-1)?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
We do provide the blocking peptide for product anti-Cytokeratin 19 Rabbit Monoclonal antibody (M02101-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-16
Question
Can you help my question with product M02101-1, anti-Cytokeratin 19 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02101-1 anti-Cytokeratin 19 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-20
Question
Do you have a BSA free version of anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1 available?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-03
Question
We ordered your anti-Cytokeratin 19 Rabbit Monoclonal antibody for WB on lower esophagus mucosa in a previous experiment. I am using mouse, and We intend to use the antibody for IF next. We are interested in examining lower esophagus mucosa as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
I have checked the website and datasheets of our anti-Cytokeratin 19 Rabbit Monoclonal antibody and I see that M02101-1 has been validated on mouse in both WB and IF. Thus M02101-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-24
Question
Will anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-03-20
Answer
According to the expression profile of cervix carcinoma erythroleukemia, KRT19 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-03-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-28
Question
My question regards using your anti-Cytokeratin 19 Rabbit Monoclonal antibody for cornification studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
We appreciate your inquiry. This M02101-1 anti-Cytokeratin 19 Rabbit Monoclonal antibody is tested on hepg2 cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-30
Question
I was wanting to use your anti-Cytokeratin 19 Rabbit Monoclonal antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
As indicated on the product datasheet, M02101-1 anti-Cytokeratin 19 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-05
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1. Please let me know if you require anything else.
B. Mitchell
Verified customer
Asked: 2017-12-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-19
Question
We have been able to see staining in mouse cervix carcinoma erythroleukemia. Do you have any suggestions? Is anti-Cytokeratin 19 Rabbit Monoclonal antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
From what I have seen in literature cervix carcinoma erythroleukemia does express KRT19. From what I have seen in Uniprot.org, KRT19 is expressed in lower esophagus mucosa, placenta, mammary gland, pancreas placenta, peripheral blood leukocyte, keratinocyte, lymph node, cervix carcinoma erythroleukemia, colon carcinoma, among other tissues. Regarding which tissues have KRT19 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Keratinocyte, Pubmed ID: 1286667
Lymph node, Pubmed ID: 10212274
Mammary gland, Pancreas, and Placenta, Pubmed ID: 15489334
Peripheral blood leukocyte, Pubmed ID: 11682035
Placenta, Pubmed ID: 2447559, 2469734, 14702039
Boster Scientific Support
Answered: 2017-08-03
Question
I see that the anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-28
Question
We are currently using anti-Cytokeratin 19 Rabbit Monoclonal antibody M02101-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
A. Carter
Verified customer
Asked: 2015-08-21
Answer
The anti-Cytokeratin 19 Rabbit Monoclonal antibody (M02101-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-08-21
Question
Is this M02101-1 anti-Cytokeratin 19 Rabbit Monoclonal antibody reactive to the isotypes of KRT19?
B. Dhar
Verified customer
Asked: 2013-01-18
Answer
The immunogen of M02101-1 anti-Cytokeratin 19 Rabbit Monoclonal antibody is A synthesized peptide derived from human Cytokeratin 19 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-01-18
