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Product Info Summary
| SKU: | M30943 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal Antibody
SKU/Catalog Number
M30943
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal Antibody (Catalog # M30943). Tested in IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30943)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: KRT6/1702
Isotype
IgG2a, kappa
Immunogen
Recombinant human keratin 6 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M30943 is reactive to in Human, Mouse
Applications
M30943 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
60045 MW
Background of
This monoclonal antibody recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Anti-Cytokeratin 6 Monoclonal Antibody (KRT6/1702)
Specific Publications For Anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal Antibody (M30943)
Hello CJ!
M30943 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Biomimetic hydrogel for rapid and scar-free healing of skin wounds inspired by the healing process of oral mucosa
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal antibody (M30943)?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
We do provide the blocking peptide for product anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal antibody (M30943). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-27
Question
Here is the WB image, lot number and protocol we used for skin using anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal antibody M30943. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal antibody M30943. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-17
Question
We want to test anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal antibody M30943 on mouse skin for research purposes, then I may be interested in using anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal antibody M30943 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
The products we sell, including anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal antibody M30943, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-21
