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Product Info Summary
| SKU: | M03692 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Cytokeratin 9 KRT9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03692
BM4647 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 9 KRT9 Rabbit Monoclonal Antibody catalog # M03692. Tested in WB, IHC, IP applications. This antibody reacts with Human Mouse Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 9 KRT9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03692)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GGH-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 9
Reactive Species
M03692 is reactive to KRT9 in Human, Mouse, Rat
Observed Molecular Weight
62 kDa
Calculated molecular weight
62.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03692 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of KRT9 using anti-KRT9 antibody (M03692).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human SIHA whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT9 antigen affinity purified monoclonal antibody (M03692) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KRT9 at approximately 62 kDa. The expected band size for KRT9 is at 62 kDa.
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IHC analysis of KRT9 using anti-KRT9 antibody (M03692).
KRT9 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KRT9 Antibody (M03692) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Cytokeratin 9 KRT9 Rabbit Monoclonal Antibody (M03692)
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4 Customer Q&As for Anti-Cytokeratin 9 KRT9 Rabbit Monoclonal Antibody
Question
We are currently using anti-Cytokeratin 9 Rabbit Monoclonal antibody M03692 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
G. Krishna
Verified customer
Asked: 2020-02-28
Answer
The anti-Cytokeratin 9 Rabbit Monoclonal antibody (M03692) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-28
Question
Do you have a BSA free version of anti-Cytokeratin 9 Rabbit Monoclonal antibody M03692 available?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytokeratin 9 Rabbit Monoclonal antibody M03692 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-20
Question
I was wanting to use to test anti-Cytokeratin 9 Rabbit Monoclonal antibody M03692 on human mammalian vulva for research purposes, then I may be interested in using anti-Cytokeratin 9 Rabbit Monoclonal antibody M03692 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Li
Verified customer
Asked: 2019-02-06
Answer
The products we sell, including anti-Cytokeratin 9 Rabbit Monoclonal antibody M03692, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-06
Question
Would M03692 anti-Cytokeratin 9 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-04
Answer
It shows on the product datasheet, M03692 anti-Cytokeratin 9 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-04
