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Product Info Summary
| SKU: | M02069 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DAZL Monoclonal Antibody
SKU/Catalog Number
M02069
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DAZL Monoclonal Antibody catalog # M02069. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DAZL Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02069)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACBF-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DAZL RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02069 is reactive to DAZL in Human
Applications
M02069 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
29 kDa
Calculated molecular weight
33.178kDa
Background of DAZL
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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Western blot analysis of DAZL expression in human testis lysate.
Protein Target Info & Infographic
Gene/Protein Information For DAZL (Source: Uniprot.org, NCBI)
Gene Name
DAZL
Full Name
Deleted in azoospermia-like
Weight
33.178kDa
Superfamily
RRM DAZ family
Alternative Names
DAZHSPGY-like-autosomal; DAZL1DAZ-like autosomal; DAZLA; Deleted in azoospermia-like 1; deleted in azoospermia-like; germline specific RNA binding protein; MGC26406; spermatogenesis gene on the Y-like autosomal; SPGYLADAZ homolog DAZL DAZH1, DAZLA, SPGYLA, DAZL deleted in azoospermia like deleted in azoospermia-like|DAZ homolog|DAZ-like autosomal|SPGY-like-autosomal|deleted in azoospermia-like 1|germline specific RNA binding protein|spermatogenesis gene on the Y-like autosomal|testis secretory sperm-binding protein Li 204a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DAZL, check out the DAZL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DAZL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DAZL Monoclonal Antibody (M02069)
Hello CJ!
No publications found for M02069
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-DAZL Monoclonal Antibody
Question
We are currently using anti-DAZL Monoclonal antibody M02069 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-DAZL Monoclonal antibody (M02069) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
My question regarding product M02069, anti-DAZL Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02069 anti-DAZL Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-10
Question
What sample was tested in WB and IHC applications with M02069?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of DAZL expression in human testis lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Is a blocking peptide available for product anti-DAZL Monoclonal antibody (M02069)?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
We do provide the blocking peptide for product anti-DAZL Monoclonal antibody (M02069). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-08
Question
Here is the WB image, lot number and protocol we used for testis using anti-DAZL Monoclonal antibody M02069. Please let me know if you require anything else.
J. Brown
Verified customer
Asked: 2016-05-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-13
