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Product Info Summary
| SKU: | A10757 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-DDA DDA1 Antibody
SKU/Catalog Number
A10757
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DDA DDA1 Antibody (Catalog # A10757). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DDA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DDA DDA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10757)
Host
Rabbit
Contents
DDA Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DDA1. The immunogen is located within the last 50 amino acids of DDA.
Reactive Species
A10757 is reactive to DDA1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
11.8 kDa
Background of DDA1
DDA1 (DET1 and DDB1 associated 1), along with DET1 and DDB1 and a member of the UBE2E group of canonical ubiquitin-conjugating enzymes, comprise DDD-E2 complexes, which interact with multiple ubiquitin E3 ligases. One of these E3 ligases is Cul4-containing E3 ligase complex CRL4. Cells depleted of DDA1 spontaneously accumulate double-stranded DNA breaks in a similar fashion as Cul4A-, Cul4B-, or WDR23-depleted cells, suggesting that DDA1 interacts with the CRL4 complex and may be involved in the ubiquitination and subsequent proteasomal degradation of target proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10757 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DDA1 antibody can be used for detection of DDA1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DDA1 in mouse heart tissue lysate with DDA1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
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Immunohistochemistry of DDA1 in mouse heart tissue with DDA1 antibody at 5 μg/mL.
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Immunofluorescence of DDA1 in mouse heart tissue with DDA1 antibody at 20 μg/mL.
Specific Publications For Anti-DDA DDA1 Antibody (A10757)
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