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Product Info Summary
| SKU: | A01374 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-DDC Antibody
SKU/Catalog Number
A01374
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DDC Antibody (Catalog # A01374). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DDC antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-DDC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01374)
Host
Rabbit
Contents
DDC Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DDC antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DDC. The immunogen is located within amino acids 390 - 440 of DDC .
Cross-reactivity
DDC antibody is human, mouse and rat reactive.
Reactive Species
A01374 is reactive to DDC in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
53.9 kDa
Background of DDC
DOPA decarboxylase (DDC) belongs to the group II decarboxylase family of proteins (1). It is an important protein in the catecholamine biosynthesis pathway. DDC catalyzes the second reaction in the biosynthesis of catecholamines, trace amines and serotonin (1,2). It can form a homodimer and is expressed in the central nervous system (2). DDC can be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease, neuroendocrine tumors and drug abuse (3,4). Defects in DDC gene may cause the autosomal recessive disorder AADC deficiency (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01374 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DDC antibody can be used for detection of DDC by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DDC in human lung tissue lysate with DDC antibody at (A) 0.5 and (B) 1 μg/ml.
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Immunohistochemistry of DDC in human lung tissue with DDC antibody at 2.5 μg/mL.
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Immunofluorescence of DDC in human lung tissue with DDC antibody at 20 μg/mL.
Specific Publications For Anti-DDC Antibody (A01374)
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