Anti-Phospho-DENND3 (Ser554) Antibody

Product Name

Anti-Phospho-DENND3 (Ser554) Antibody

View all DENND3 Antibodies

SKU/Catalog Number

P13588

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-DENND3 (Ser554) Antibody (Catalog # P13588). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-DENND3 (Ser554) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P13588)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 of human DENND3, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P13588 is reactive to DENND3 in Human

Applications

P13588 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

135.89kDa

Background of DENND3

The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (Marat, A.L., et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (Xu and McPherson, 2015; Matsui et al., 2011). ULK-mediated phosphorylation of DENND3 at serines 554 and 572 upregulates its GEF activity toward the small GTPase Rab12 (Xu and McPherson, 2015).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500

Validation Images & Assay Conditions

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~142 kDa DENND3 protein phosphorylated at Ser⁵⁵⁴ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (gamma-Ptase, 800 units/1mg protein for 30 min).

Gene/Protein Information For DENND3 (Source: Uniprot.org, NCBI)

Alternative Names

DENN/MADD domain containing 3; KIAA0870DENN domain-containing protein 3 DENND3 DENN domain containing 3 DENN domain-containing protein 3|DENN/MADD domain containing 3

For more info on DENND3, check out the DENND3 Infographic

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In this infographic, you will see the following information for DENND3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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