Product Info Summary
SKU: | MA1036 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rabbit, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-Desmin Antibody (Monoclonal, DE-U-10)
SKU/Catalog Number
MA1036
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Desmin Antibody (Monoclonal, DE-U-10) catalog # MA1036. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Desmin Antibody (Monoclonal, DE-U-10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1036)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
DE-U-10
Isotype
Mouse IgG1
Immunogen
Desmin from pig stomach.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1036 is reactive to DES in Human, Mouse, Rabbit, Rat
Applications
MA1036 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
53 kDa
Calculated molecular weight
53.536kDa
Background of Desmin
Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball (1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al. (1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rat, rabbit, By Heat
Western blot, 2μg/ml, Human, mouse, rat, rabbit
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Desmin using anti-Desmin antibody (MA1036).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.
Lane 1: Rat Skeletal Muscle Tissue Lysate,
Lane 2: Rat Cardiac Muscle Tissue Lysate,
Lane 3: Mouse Skeletal Muscle Tissue Lysate,
Lane 4: Mouse Cardiac Muscle Tissue Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Desmin antigen affinity purified monoclonal antibody (Catalog # MA1036) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Desmin at approximately 53 kDa. The expected band size for Desmin is at 53 kDa.
Click image to see more details
Figure 2. IHC analysis of Desmin using anti-Desmin antibody (MA1036).
Desmin was detected in a paraffin-embedded section of Rat Cardiac Muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Desmin Antibody (MA1036) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Desmin using anti-Desmin antibody (MA1036).
Desmin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Desmin Antibody (MA1036) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Desmin using anti-Desmin antibody (MA1036).
Desmin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Desmin Antibody (MA1036) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Desmin using anti-Desmin antibody (MA1036).
Desmin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Desmin Antibody (MA1036) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Desmin using anti-Desmin antibody (MA1036).
Desmin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Desmin Antibody (MA1036) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of Desmin using anti-Desmin antibody (MA1036).
Desmin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Desmin Antibody (MA1036) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For DES (Source: Uniprot.org, NCBI)
Gene Name
DES
Full Name
Desmin
Weight
53.536kDa
Superfamily
intermediate filament family
Alternative Names
CMD1I; CMD1IFLJ41013; CSM1; CSM2; DES; Desmin; FLJ12025; FLJ39719; FLJ41793; intermediate filament protein DES CDCD3, CSM1, CSM2, LGMD1D, LGMD1E, LGMD2R desmin desmin|cardiomyopathy, dilated 1F (autosomal dominant)|epididymis secretory sperm binding protein|intermediate filament protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DES, check out the DES Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DES: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Desmin Antibody (Monoclonal, DE-U-10) (MA1036)
Hello CJ!
MA1036 has been cited in 21 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Interleukin-10 promotes primary rat hepatic stellate cell senescence by upregulating the expression levels of p53 and p21
Identification of apoptosis-related microRNAs and their target genes in myocardial infarction post-transplantation with skeletal myoblasts
Liu J, Wang Y, Du W, Liu W, Liu F, Zhang L, Zhang M, Hou M, Liu K, Zhang S, Yu B. Plos One. 2013;8(3):E58883. Doi: 10.1371/Journal.Pone.0058883. Epub 2013 Mar 22. Wnt1 Inhibits Hydrogen Peroxide-Induced Apoptosis In Mouse Cardiac Stem Cells.
Wang W, Fan M, Zhang L, Liu Sh, Sun L, Wang Cy. Biomed Mater. 2009 Apr;4(2):025011. Doi: 10.1088/1748-6041/4/2/025011. Epub 2009 Mar 4. Compatibility Of Hyaluronic Acid Hydrogel And Skeletal Muscle Myoblasts.
Zhang F, Deng B, Wen J, Chen K, Liu W, Ye S, Huang H, Jiang S, Xiong Y. Biochem Biophys Res Commun. 2015 Mar 6;458(2):375-80. Doi: 10.1016/J.Bbrc.2015.01.120. Epub 2015 Jan 31. Ppar?? And Myod Are Differentially Regulated By Myostatin In Adipose-D...
Liu Hh, Li L, Chen X, Cao W, Zhang Rp, Yu Hy, Xu F, He H, Wang Jw. Cytotechnology. 2011 Aug;63(4):399-406. Doi: 10.1007/S10616-011-9356-7. Epub 2011 May 26. Characterization Of In Vitro Cultured Myoblasts Isolated From Duck (Anas Platyrhynchos) Em...
Chen S, Fang Z, Zhu Z, Deng A, Liu J, Zhang C. J Huazhong Univ Sci Technolog Med Sci. 2009 Dec;29(6):715-9. Doi: 10.1007/S11596-009-0608-0. Epub 2009 Dec 29. Protective Effect Of Sulodexide On Podocyte Injury In Adriamycin Nephropathy Rats.
Liu J, Liu Y, Xu Y, Wang M, Zhu Q, Li W, Wang X, Liu J, Ma B, Wu K. Exp Mol Pathol. 2013 Feb;94(1):79-83. Doi: 10.1016/J.Yexmp.2012.10.012. Epub 2012 Oct 23. Neurotransmitter Noradrenaline Downregulate Cytoskeletal Protein Expression Of Vsmcs.
Sondag Gr, Salihoglu S, Lababidi Sl, Crowder Dc, Moussa Fm, Abdelmagid Sm, Safadi Ff. J Cell Physiol. 2014 Jul;229(7):955-66. Doi: 10.1002/Jcp.24512. Osteoactivin Induces Transdifferentiation Of C2C12 Myoblasts Into Osteoblasts.
Zhang Sc, Zheng Yh, Yu Pp, Min Th, Yu Fx, Ye C, Xie Yk, Zhang Qy. World J Gastroenterol. 2012 Jul 28;18(28):3696-704. Doi: 10.3748/Wjg.V18.I28.3696. Lentiviral Vector-Mediated Down-Regulation Of Il-17A Receptor In Hepatic Stellate Cells Results In...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Desmin Antibody (Monoclonal, DE-U-10)
Question
Do you have a BSA free version of anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 available?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-14
Question
Is this MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) reactive to the isotypes of DES?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
The immunogen of MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) is Desmin from pig stomach. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-06
Question
Is a blocking peptide available for product anti-Desmin antibody (Monoclonal, DE-U-10) (MA1036)?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
We do provide the blocking peptide for product anti-Desmin antibody (Monoclonal, DE-U-10) (MA1036). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagogastric junction muscularis propria using anti-Desmin antibody (Monoclonal, DE-U-10) MA1036. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-25
Question
I was wanting to use to test anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 on human esophagogastric junction muscularis propria for research purposes, then I may be interested in using anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Edwards
Verified customer
Asked: 2019-08-20
Answer
The products we sell, including anti-Desmin antibody (Monoclonal, DE-U-10) MA1036, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-20
Question
I would like using your anti-Desmin antibody (Monoclonal, DE-U-10) for r-rno-390522; striated muscle contraction studies. Has this antibody been tested with western blotting on cardiac muscle tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
I appreciate your inquiry. This MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) is tested on rat skeletal muscle tissue, cardiac muscle tissue, tissue lysate, mouse skeletal muscle tissue. It is guaranteed to work for IHC, WB in human, mouse, rabbit, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-27
Question
My team were happy with the WB result of your anti-Desmin antibody (Monoclonal, DE-U-10). However we have observed positive staining in muscle myofibril using this antibody. Is that expected? Could you tell me where is DES supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
From literature, muscle does express DES. Generally DES expresses in cytoplasm, myofibril, sarcomere, z line. Regarding which tissues have DES expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 8792816, 15489334
Skeletal muscle, Pubmed ID: 10545598
Boster Scientific Support
Answered: 2019-06-21
Question
Would anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 work for WB with esophagogastric junction muscularis propria?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
According to the expression profile of esophagogastric junction muscularis propria, DES is highly expressed in esophagogastric junction muscularis propria. So, it is likely that anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 will work for WB with esophagogastric junction muscularis propria.
Boster Scientific Support
Answered: 2019-06-20
Question
I see that the anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-22
Question
See attached the WB image, lot number and protocol we used for esophagogastric junction muscularis propria using anti-Desmin antibody (Monoclonal, DE-U-10) MA1036. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-01
Question
My question regarding product MA1036, anti-Desmin antibody (Monoclonal, DE-U-10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1036 anti-Desmin antibody (Monoclonal, DE-U-10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-15
Question
We are currently using anti-Desmin antibody (Monoclonal, DE-U-10) MA1036 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rabbit, rat. Is it true that the antibody can work on feline tissues as well?
L. Miller
Verified customer
Asked: 2017-09-01
Answer
The anti-Desmin antibody (Monoclonal, DE-U-10) (MA1036) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-01
Question
I was wanting to use your anti-Desmin antibody (Monoclonal, DE-U-10) for WB for human esophagogastric junction muscularis propria on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human esophagogastric junction muscularis propria identification?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
It shows on the product datasheet, MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) has been tested for IHC, WB on human, mouse, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in human esophagogastric junction muscularis propria in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-21
Question
Our lab used your anti-Desmin antibody (Monoclonal, DE-U-10) for IHC on muscle in a previous project. I am using rabbit, and We intend to use the antibody for WB next. My lab would like examining muscle as well as esophagogastric junction muscularis propria in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
K. Carter
Verified customer
Asked: 2016-11-23
Answer
I viewed the website and datasheets of our anti-Desmin antibody (Monoclonal, DE-U-10) and I see that MA1036 has been tested on rabbit in both IHC and WB. Thus MA1036 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rabbit even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-11-23
Question
We have been able to see staining in rat liver. Any tips? Is anti-Desmin antibody (Monoclonal, DE-U-10) supposed to stain liver positively?
K. Li
Verified customer
Asked: 2015-11-02
Answer
From what I have seen in literature liver does express DES. From what I have seen in Uniprot.org, DES is expressed in esophagogastric junction muscularis propria, muscle, skeletal muscle, liver, among other tissues. Regarding which tissues have DES expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 8792816, 15489334
Skeletal muscle, Pubmed ID: 10545598
Boster Scientific Support
Answered: 2015-11-02
Question
Would MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Zhang
Verified customer
Asked: 2013-11-13
Answer
It shows on the product datasheet, MA1036 anti-Desmin antibody (Monoclonal, DE-U-10) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-11-13