Anti-Desmoglein 2/DSG2 Antibody
Rabbit IgG polyclonal antibody for Desmoglein-2(DSG2) detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
Anti-Desmoglein 2/DSG2 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||Flow Cytometry, IF, IHC-P, ICC, WB|
|Product Name||Anti-Desmoglein 2/DSG2 Antibody
See all Desmoglein-2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for DESMOGLEIN 2/DSG2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. DESMOGLEIN 2/DSG2 information: Molecular Weight: 122294 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome; Tissue Specificity: All of the tissues tested and carcinomas.|
|Cite This Product||Anti-Desmoglein 2/DSG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1559)|
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|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||Flow Cytometry, IF, IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Desmoglein 2(1104-1118aa STRVTKHSTVQHSYS), different from the related mouse and rat sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For DSG2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||1829|
|Species Of This Entry||Human|
|Alternative Names||Desmoglein-2|ARVC10; ARVD10; Cadherin family member 5; CDHF5; CDHF5MGC117034; CMD1BB; desmoglein 2; Desmoglein2; Desmoglein-2; DSG2; HDGC; MGC117036; MGC117037|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Oxidation|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome.|
|Gene Location||18q12.1, on Chromasome 18, gene sequence: NC_000018.10 (31498177..31549008)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For DSG2 (Source: Uniprot.org, NCBI)
|Protein Function||Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.|
|Tissue Specificity||All of the tissues tested and carcinomas.|
|Related Diseases||Tissue Adhesions; Arrhythmogenic Right Ventricular Dysplasia; Neoplasms; Cardiomyopathies; Malignant Neoplasms; Carcinoma; Cardiac Arrhythmia; Dental Plaque; Pemphigus Foliaceus; Neoplasm Metastasis; Malignant Squamous Cell Neoplasm; Sudden Death; Bulla; Skin Neoplasms; Malignant Paraganglionic Neoplasm; Cell Invasion; Sudden Cardiac Death; Dermatologic Disorders|
|Related Pathways||Cell Adhesion; Cell-cell Adhesion; Pathogenesis; Localization; Desmosome Assembly; Cell Proliferation; Cell Migration; Cell Death; Keratinization; Transport; Keratinocyte Differentiation; Inflammatory Response; Epithelial Cell-cell Adhesion; Hair Follicle Development; Programmed Cell Death; Proteolysis; Immune Response; Tropism; Epiboly; Spermatogenesis|
|Background||Desmoglein-2 is a protein that in humans is encoded by the DSG2 gene. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in DSG2 display a high degree of penetrance. Disease expression was of variable severity with LV involvement a prominent feature. The low prevalence of classical ECG changes highlights the need to expand current diagnostic criteria to take account of LV disease, childhood disease expression, and incomplete penetrance.|
|Scientific References||PMID: 8143788 by Schaefer S., et al. Identification of the ubiquitous human desmoglein, Dsg2, and the expression catalogue of the desmoglein subfamily of desmosomal cadherins.|
PMID: 1935985 by Koch P.J., et al. Complete amino acid sequence of the epidermal desmoglein precursor polypeptide and identification of a second type of desmoglein gene.
Our Boster Quality Guarantee for Anti-Desmoglein 2/DSG2 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human SW620 whole cell lysates,
Lane 7: rat liver tissue lysates,
Lane 8: mouse liver tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Desmoglein 2 antigen affinity purified polyclonal antibody (Catalog # PA1559) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmoglein 2 at approximately 160KD. The expected band size for Desmoglein 2 is at 122KD.
DSG2 was detected in paraffin-embedded section of human breast carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DSG2 Antibody (PA1559) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
DSG2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DSG2 Antibody (PA1559) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
DSG2 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DSG2 Antibody (PA1559) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
DSG2 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DSG2 Antibody (PA1559) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Overlay histogram showing 293T cells stained with PA1559 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DSG2 Antibody (PA1559,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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