Anti-DGKI/Dgk Iota Antibody

DGK-iota antibody

Boster Bio Anti-DGKI/Dgk Iota Antibody (Catalog # A11534). Tested in WB, IF applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: A11534
Size: 100 µl
Reactive Species: Human, Rat
Host: Rabbit
Application: IF, WB

Product Name

Anti-DGKI/Dgk Iota Antibody

View all DGK-iota Antibodies

SKU/Catalog Number

A11534

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-DGKI/Dgk Iota Antibody (Catalog # A11534). Tested in WB, IF applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DGKI/Dgk Iota Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11534)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from C-terminal of human DGKI.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11534 is reactive to DGKI in Human, Rat

Applications

A11534 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

116.997kDa

Background of DGK-iota

This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.

Ding L., J. Biol. Chem. 273:32746-32752(1998).
Bowne S.J., Mol. Vis. 6:6-9(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For DGKI (Source: Uniprot.org, NCBI)

Gene Name

DGKI

Full Name

Diacylglycerol kinase iota

Weight

116.997kDa

Superfamily

eukaryotic diacylglycerol kinase family

Alternative Names

DAG kinase iota; DGKI; DGKiota; DGK-iota; diacylglycerol kinase iota; diacylglycerol kinase, iota; Diglyceride kinase iota; EC 2.7.1.107 DGKI DGK-IOTA diacylglycerol kinase iota diacylglycerol kinase iota|DAG kinase iota|diglyceride kinase iota

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DGKI, check out the DGKI Infographic

DGKI infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DGKI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-DGKI/Dgk Iota Antibody

Question

My question regards to test anti-DGKI/Dgk Iota antibody A11534 on human retina for research purposes, then I may be interested in using anti-DGKI/Dgk Iota antibody A11534 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

The products we sell, including anti-DGKI/Dgk Iota antibody A11534, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-30

Question

We are currently using anti-DGKI/Dgk Iota antibody A11534 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

The anti-DGKI/Dgk Iota antibody (A11534) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-19

Question

Is a blocking peptide available for product anti-DGKI/Dgk Iota antibody (A11534)?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

We do provide the blocking peptide for product anti-DGKI/Dgk Iota antibody (A11534). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-05

Question

Will anti-DGKI/Dgk Iota antibody A11534 work on horse IF with putamen?

Verified Customer

Verified customer

Asked: 2019-01-28

Answer

Our lab technicians have not tested anti-DGKI/Dgk Iota antibody A11534 on horse. You can run a BLAST between horse and the immunogen sequence of anti-DGKI/Dgk Iota antibody A11534 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse putamen in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-28

Question

Is this A11534 anti-DGKI/Dgk Iota antibody reactive to the isotypes of DGKI?

Verified Customer

Verified customer

Asked: 2018-03-15

Answer

The immunogen of A11534 anti-DGKI/Dgk Iota antibody is Synthesized peptide derived from C-terminal of human DGKI. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-03-15

Question

Please see the WB image, lot number and protocol we used for retina using anti-DGKI/Dgk Iota antibody A11534. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-10-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-12

Order DetailsPrice
A11534-100uL

100uL

$360

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A11534
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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