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Product Info Summary
| SKU: | M02248 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Dkk-3 Monoclonal Antibody
SKU/Catalog Number
M02248
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Dkk-3 Monoclonal Antibody catalog # M02248. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dkk-3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02248)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
4G7
Isotype
IgG
Immunogen
Purified recombinant fragment of human Dkk-3 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02248 is reactive to DKK3 in Human, Monkey
Applications
M02248 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
38.39kDa
Background of Dkk-3
LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 2. Flow Cytometry validation of DKK3 using Anti-Dkk-3 Monoclonal Antibody (M02248).
Flow cytometric (FCM) analysis of MCF-7 cells using Dkk-3 Monoclonal Antibody (green) and negative control (purple).
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Figure 1. Western blotting validation for Anti-Dkk-3 Monoclonal Antibody M02248
Western Blot (WB) analysis using Dkk-3 Monoclonal Antibody against HEK293 (1)Protein Target Info & Infographic
Gene/Protein Information For DKK3 (Source: Uniprot.org, NCBI)
Gene Name
DKK3
Full Name
Dickkopf-related protein 3
Weight
38.39kDa
Superfamily
dickkopf family
Alternative Names
dickkopf (Xenopus laevis) homolog 3; dickkopf 3; dickkopf homolog 3 (Xenopus laevis); Dickkopf-3; dickkopf-related protein 3; Dkk3; Dkk-3; hDkk-3; regulated in glioma; REIC; REICdkk-3; RIG; RIG-like 5-6; RIG-like 7-1 DKK3 REIC, RIG dickkopf WNT signaling pathway inhibitor 3 dickkopf-related protein 3|RIG-like 5-6|RIG-like 7-1|dickkopf 3 homolog|dickkopf homolog 3|dickkopf-3|dkk-3|hDkk-3|regulated in glioma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DKK3, check out the DKK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DKK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dkk-3 Monoclonal Antibody (M02248)
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No publications found for M02248
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Customer Reviews
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